黑素瘤缺乏因子2在乙型肝炎病毒相关性肾小球肾炎发病机制中的作用  

The role of absent in melanoma 2 in the pathogenesis of hepatitis B virus associated glomerulonephritis

作　　者：马书敏[1] 杜文军[2] 郑昭敏[2] 甄军晖[3] 陈士俊[2] 

机构地区：[1]山东大学医学院在读研究生,济南250012 [2]山东大学附属济南市传染病医院介入中心 [3]山东大学附属齐鲁医院病理科

出　　处：《中华传染病杂志》2012年第4期226-230,共5页Chinese Journal of Infectious Diseases

基　　金：济南市科技局资助项目（201101116）

摘　　要：目的初步探讨黑素瘤缺乏因子2（AIM2）激活与表达在HBV相关性肾小球肾炎（HBV—GN）发病机制中的作用。方法54例HBV—GN患者为研究组，25例慢性肾小球肾炎患者为阴性对照组，6例慢性乙型肝炎患者为阳性对照组。免疫组织化学法分别测定研究组、阴性对照组患者肾组织及阳性对照组患者肝组织中AIM2、Caspase-1、IL-1β的表达。组间比较采用卡方检验，相关性分析采用Spearman相关分析。结果54例HBV-GN患者肾组织中AIM2的阳性表达率为81．4％，25例慢性肾小球肾炎组为4．0％，两组间差异有统计学意义（X2=38．746，P〈0．01）。AIM2在6例慢性乙型肝炎患者肝组织中均有表达。HBV—GN组AIM2的表达强度与Caspase-1呈正相关（rs=0．444，P〈0．01），IL-1β的表达强度与Caspase-1也呈正相关（rs=0．515，P〈0．01）。HBV-GN患者中血HBVDNA≥1×10^5拷贝／mL者AIM2的表达强度高于HBVDNA〈1×10^5拷贝／mL者（X2=6．097，P〈0．05）。结论AIM2的激活与HBV—GN有关，可能通过Caspase一1激活，释放炎性因子IL-1β。Objective To explore the role of activation and expression of absent in melanoma 2 （AIM2） in pathogenesis of hepatitis B virus （HBV） associated glomerulonephritis （HBV-GN）. Methods Fifty-four patients with HBV-GN were recruited into the investigational group, and 25 patients with chronic glomerulonephritis （CGN） and 6 patients with chronic hepatitis B （CHB） were included as negative control group and positive control group, respectively. Immunohistochemistry was used to detect AIM2, Caspase-1 and interleukin （IL）-1β expressions in both kidney tissues from the investigational group and negative control group and liver tissues from positive control group. The differences between groups were compared by chi-square test, and the correlation analysis was conducted by Spearman correlation test. Results The positive expression rates of AIM2 in 54 patients of HBV-GN group and 25 of CGN group were 81.4% and 4.0%, respectively （X2= 38. 746, P〈 0.01）. AIM2 was found to be expressed in all 6 CHB patients. In HBV-GN group, the expression of AIM2 was positively correlated with Caspase-1 （rs = 0. 444, P〈0.01）, and the expression of IL-1β was also positively correlated with Caspase-1 （rs =0. 515 ,P〈0.01）. In HBV-GN group, the expression of AIM2 in patients with HBV DNA≥1 × 10^5 copy/mL was significantly higher than that in those with HBV DNA〈1 × 10^5copy/mL （X2 =6. 097, P〈0.05）. Conclusion The activation of AIM2 may play a role in the pathogenesis of HBV-GN through Caspase-1 activation and inflammatory factor IL-1β release.

关 键 词：肝炎病毒 乙型 肾小球肾炎 半胱氨酸天冬氨酸蛋白酶1 白细胞介素1Β 

分 类 号：R51[医药卫生—内科学] R692[医药卫生—临床医学]