猪SFRP4基因的表达规律及抑制剂SP600125对前体脂肪细胞分化的影响  

Expression of Porcine SFRP4 and Its Effect on Differentiation of Preadipocytes via Inhibitor SP600125

作　　者：关华[1] 郑加盟[1] 陈宗正[1] 宋子仪[1] 杨浩[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物科技学院脂肪沉积与肌肉发育实验室,陕西杨凌712100

出　　处：《中国生物化学与分子生物学报》2012年第5期455-463,共9页Chinese Journal of Biochemistry and Molecular Biology

基　　金：转基因生物新品种培育重大专项(No.2009ZX08009-157B)~~

摘　　要：分泌型卷曲相关蛋白4(secreted frizzled-related protein 4,SFRP4)是Wnt信号通路可溶解的调控子.通过高通量测序(Solexa)技术、实时定量PCR(RT-qPCR)技术对瘦肉型和脂肪型猪不同生长阶段脂肪组织、脂肪细胞中SFRP4表达规律进行研究;用JNK信号通路特异性抑制剂SP600125处理猪原代前体脂肪细胞,研究抑制JNK信号通路对猪前体脂肪分化以及SFRP4 mRNA和蛋白表达的影响.结果显示,SFRP4在脂肪型猪脂肪组织表达量显著高于瘦肉型猪(P<0.01);不同组织检测结果发现,SFRP4广泛表达于各个组织,并高表达于脂肪组织;前体脂肪细胞向成熟脂肪细胞分化过程中SFRP4表达量逐渐升高;SP600125促进前体脂肪细胞分化,引起PPARγ、FABP4、ATGL、Perilipin的显著升高(P<0.01),而SFRP4表达被显著抑制.本研究为调控脂肪细胞分化关键基因的筛选提供新的理论参考.Secreted frizzled-related protein 4（SFRP4） was the regulator of Wnt signaling pathway.The expression of SFRP4 in fat tissues of different genotype pigs and in different growth periods were explored by high-throughout sequencing（Solexa） and real-time quantity PCR（RT-qPCR）.The effect on differentiation of preadipocyte and the expression level of SFRP4 were explored by inhibitor SP600125 in JNK signaling pathway.The results showed that expression level of SFRP4 was higher in fat tissues of obese-type than that in the lean-type.Expression level of SFRP4 in the porcine fat tissue and adipocyte was influenced by ages,and was higher in fat,but lower in other tissues.SP600125 promoted the adiocytes to differentiate and significantly increased expression levels of PPARγ,FABP4,ATGL,Perilipin,but the SFRP4 expression level was inhibited significantly.This research provides a new reference for screen the key genes in adipocyte differentiation.

关 键 词：分泌型卷曲相关蛋白4 高通量测序 前体脂肪细胞 特异性抑制剂SP600125 分化 

分 类 号：Q78[生物学—分子生物学] Q246