乳酸锌对猪空肠上皮细胞IPEC-J2增殖及相关调控基因mRNA表达的影响  

Effect of Zinc Lactate on Cell Proliferation and Related-regulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPEC-J2

作　　者：韩国全[1] 余冰[1,2] 陈代文[1,2] 相振田[1] 亓宏伟[1] 陈洪[1] 毛倩[1] 毛湘冰[1] 黄志清[1] 

机构地区：[1]四川农业大学动物营养研究所,雅安625014 [2]四川农业大学动物抗病营养教育部重点实验室,雅安625014

出　　处：《畜牧兽医学报》2012年第5期740-747,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：教育部长江学者和创新团队发展计划项目资助(IRTO555);现代农业产业技术体系建设专项资金资助(CARS-36);博士后资金资助(04310610)

摘　　要：旨在探讨乳酸锌对猪空肠上皮细胞增殖及相关调控基因ZnT2、DMT1、IREG-1、MT1和ZIP4mRNA表达的影响。用乳酸锌的锌浓度分别为50、100、150、200mg.L-1的培养基培养IPEC-J2细胞,采用比色法测定分析细胞增殖变化;用实时荧光定量RT-PCR方法检测ZnT2、DMT1、IREG-1、MT1及ZIP4mRNA表达,以TBPmRNA的表达水平作为内参对照。在细胞培养前36h,乳酸锌对IPEC-J2细胞基本没有影响,随锌浓度递增细胞增殖幅度升高;乳酸锌处理IPEC-J2细胞后,ZnT2、DMT1、IREG-1及MT1mRNA表达随锌浓度增高而升高,ZIP4mRNA表达则随锌浓度增高而降低。添加乳酸锌可以促进IPEC-J2细胞增殖,上调ZnT2、DMT1、IREG-1、MT1mRNA表达,下调ZIP4mRNA表达。The present study was conducted to evaluate the effects of zinc lactate on cell proliferation of jejunal epithelial cells IPEC-J2 and mRNA expression of related-regulatory genes in porcine.IPEC-J2 cells were cultured with mediums added with zinc lactate by the zinc concentrations of 50,100,150 and 200 mg·L-1,respectively.Cell proliferation was estimated by Colorimetric analysis.Simultaneously,real-time quantitative RT-PCR was applied to detect the mRNA expression of related-regulatory genes（ZnT2,DMT1,IREG-1,MT1 and ZIP4）,and TBP mRNA level was used as the control.During the first 36 hours after treatment with zinc lactate,there was almost no effect on IPEC-J2 cell proliferation.Subsequently,with increasing of the concentration of zinc,the rate of cell proliferation gradually increased.At the same time,after IPEC-J2 cell was treated with zinc lactate,the expression of ZnT2,DMT1,IREG-1and MT1 mRNA was increased with the increase of the concentration of zinc while the expression of ZIP4 mRNA decreased.Zinc lactate could promote cell proliferation of IPEC-J2,up-regulated the mRNA expression of ZnT2,DMT1,IREG-1,MT1 and down-regulated the mRNA expression of ZIP4.

关 键 词：乳酸锌 猪空肠上皮细胞IPEC-J2 MRNA表达 相关调控基因 

分 类 号：S828[农业科学—畜牧学] S815.4[农业科学—畜牧兽医]