Wnt3a在不同毛色羊驼皮肤中的表达和定位  

Expression and Localization of Wnt3a in Different Colors of Alpaca Skin

作　　者：宋云飞[1] 田雪[1] 卢绪秀[1] 郭青云[1] 张丹丽[1] 解晓晶[1] 于秀菊[1] 贺俊平[1] 王海东[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2012年第5期785-790,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山西省研究生优秀创新项目(20113058)

摘　　要：本研究旨在探索Wnt3a在羊驼皮肤中的表达与定位。以不同毛色的成年羊驼为研究对象,应用荧光定量PCR技术分析不同毛色羊驼皮肤Wnt3a基因的相对表达量,并运用Western blotting及免疫组织化学法对Wnt3a蛋白在不同毛色羊驼皮肤中进行表达和定位研究。结果:荧光定量PCR结果显示棕色羊驼中Wnt3amRNA相对表达量是白色羊驼的2.970 2倍;Western blotting结果表明,羊驼皮肤组织组蛋白提取物中存在相对分子质量约39ku的产物,棕色羊驼皮肤平均蛋白表达量显著高于白色羊驼;免疫组织化学结果显示Wnt3a在羊驼皮肤毛囊的根鞘和毛球部呈阳性表达,根据光密度值分析得出Wnt3a在棕色和白色羊驼毛囊中的表达差异显著(P<0.05)。通过以上研究显示Wnt3a可能与羊驼毛色形成具有相关性。This experiment was conducted to explore the expression and localization of Wnt3a in different colors of alpaca skin.The tissues were obtained from white and brown adult alpacas skins.The comparative expression quantity of gene Wnt3a in different coat colors was analyzed by real time quantitative PCR.The protein expression and localization of Wnt3a were measured by Western blotting and immunohistochemistry.The results of QRT-PCR showed that the comparative expression quantity of gene Wnt3a in brown alpacas was 2.970 2 times than that in white ones.The Western blotting results showed that the protein expression of wnt3a in brown alpaca skins was significantly higher than that in white alpaca.The positive signals of Wnt3a were found in hair bulb and outer root sheath of hair follicle,and the expression of Wnt3a was significantly different between the white and brown alpaca skin based on the average optical density（P0.05）.Our findings showed that Wnt3a may involved in the regulation of hair color formation.

关 键 词：WNT3A 羊驼 皮肤 免疫组化 荧光定量PCR Westernblotting 

分 类 号：S852.16[农业科学—基础兽医学]