子宫内膜基质金属蛋白酶-2、-9 mRNA表达与奶牛子宫内膜炎关系的研究  

The Relationship between MMP-2,MMP-9 mRNA of Bovine Endometrium and Endometritis

作　　者：王国卿[1,2] 王洪海 张乃生[2] 

机构地区：[1]东北大学生物技术研究所,沈阳110004 [2]吉林大学畜牧兽医学院,长春130062 [3]大庆市畜牧兽医局,大庆163311

出　　处：《畜牧兽医学报》2012年第5期810-815,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中国博士后科学基金(200902492);中央高校基本科研业务费(N110405008)

摘　　要：为探讨基质金属蛋白酶(MMPs)-2、MMP-9在奶牛子宫内膜炎中的作用机制,选用产后6～10d健康及患急性化脓性子宫内膜炎的中国荷斯坦奶牛各10头,分别为对照组和试验组,通过ELISA检测PGF2a、孕酮浓度;免疫组化检测子宫内膜Ⅰ型胶原、Ⅳ型胶原的表达;SYBR Green I荧光定量PCR检测子宫内膜MMP-2和MMP-9mRNA表达。结果表明:试验组PGF2a浓度极显著低于对照组(P<0.01),孕酮含量显著高于对照组(P<0.05),PGF2a与孕酮浓度呈显著负相关(r=0.893);试验组子宫内膜中Ⅰ型胶原、Ⅳ型胶原的表达量均高于对照组,差异极显著(P<0.01);MMP-2和MMP-9mRNA水平则显著低于对照组(P<0.01)。结果提示:产后子宫内膜炎病牛子宫内膜MMP-2、MMP-9低表达,导致Ⅰ型和Ⅳ型胶原降解受阻,使细胞外基质为子宫内膜细胞提供的刺激信号不足,子宫分泌PGF2a量少,黄体消退障碍,血清孕酮含量高;说明子宫内膜MMP-2、MMP-9mRNA表达对产后子宫内膜炎的发生和发展起促进作用。The study was conducted to investigate the role of MMP-2 and MMP-9 in cow endometritis.Healthy and suffering from acute purulent endometritis Chinese Holstein cows were selected as control and experimental group respectively with 10 in each group and all of the cows were 6-10 days postpartum.The concentration of PGF2a,Progesterone was detected by ELISA.CollagenⅠand Ⅳ in endometrium were checked by Immunohistochemistry.MMP-2 and MMP-9 expression were examined using real-time PCR in the endometrium.The results showed that the concentration of PGF2a in experimental group was lower than that in control group（P0.01）.The experimental group progesterone was significantly higher than the control group.The expression level of CollagenⅠand Ⅳ in endometristis was higher than the control group（P0.01）.The MMP-2 and MMP-9 mRNA expression in endometristis were lower than the control group（P0.01）.Conclusion indicated that the expression of MMP-2 and MMP-9 mRNA in cow endometristis reduced,CollagenⅠand Ⅳ degradation were blocked and endometrial cell was unable to secrete PGF2a,making the concentration of progesterone increase in blood serum.MMP-2 and MMP-9 mRNA influenced postpartum endometrial function and encouraged the development of postpartum endometritis.

关 键 词：奶牛 子宫内膜炎 MMPS 荧光定量PCR 胶原 

分 类 号：S857.23[农业科学—临床兽医学]