芩连液与白虎汤对气分证家兔补体经典途径活化的影响比较  

Effect of Baihu Soup and Qinlian Liquid on the Classical Pathway of Complement Activation in Rabbit with Qifen Syndrome

作　　者：张世栋[1] 王东升[1] 王旭荣[1] 李世宏[1] 李锦宇[1] 陈炅然[1] 李宏胜[1] 严作廷[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,中国农业科学院临床兽医学研究中心,甘肃省中兽药工程技术研究中心,兰州730050

出　　处：《畜牧兽医学报》2012年第5期816-820,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十一五"国家科技支撑计划重点课题(2008BADB4B01-2)

摘　　要：为比较经方白虎汤与自拟方芩连液的分子疗效区别,以静脉注射内毒素建立了具有高热证候的气分证家兔模型,并用白虎汤和自拟方芩连液分别进行治疗,通过ELISA检测比较治疗前后血清总补体(CH50)的含量变化,并采用实时荧光定量PCR技术检测了肝组织中补体第三成分(C3)基因和C-反应蛋白(CRP)基因的表达变化。结果表明,在气分证家兔模型中,补体系统以经典途径活化。白虎汤的治疗能同时保证C3基因和CRP基因的高表达起到治愈作用,而芩连液仅以提高CRP的表达量发挥一定的保护作用。研究表明白虎汤对动物气分证的疗效优于芩连液。The objective of this study was to compare the curative effect of Baihu Soup with Qinlian Liquid in molecular angle.The animal model of Qifen Syndrome was found through intravenous（i.v.） injection of LPS,and the model was treated with Baihu Soup and Qinlian Liquid respectively.Before and after treatment,the expression changes of serum CH50,C3 and CRP gene in liver tissue were determined by ELISA and real-time quantitative PCR respectively.The results demonstrated that complement system was activated through classical pathway.Baihu Soup can cured the animals through make genes of C3 and CRP high expression,and Qinlian Liquid plays a certain role only through up-regulated the gene expression of CRP.The curative effect of Baihu Soup on animal Qifen Syndrome is superior to that of Qinlian Liquid.

关 键 词：气分证 补体 CH50 C3 CRP 

分 类 号：S853.1[农业科学—临床兽医学]