骨骼肌特异性敲除TβRⅡ小鼠模型的建立  

Construction of the skeletal muscle-specific TβRⅡ knockout mice

作　　者：王旭[1,2] 王晶[3] 王璐[3] 王华旻[4] 管又飞[4] 范明[1] 陈晓萍[3] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]沈阳军区总医院医学实验科,辽宁沈阳110840 [3]中国航天员科研训练中心,北京100094 [4]北京大学医学部生理与病理生理学系,北京100191

出　　处：《中国应用生理学杂志》2012年第3期284-287,共4页Chinese Journal of Applied Physiology

摘　　要：目的:建立骨骼肌特异性敲除转化生长因子受体Ⅱ(TβRⅡ)小鼠模型,为进一步研究TβRⅡ在骨骼肌发育和分化中的作用奠定基础。方法:首先将TβRⅡflox/flox转基因小鼠与上游携带肌酸激酶(MCK)启动子的MCK-Cre转基因小鼠进行杂交,培育繁殖出TβRⅡflox/wt/MCK-Cre(+)双转基因小鼠。然后利用TβRⅡflox/wt/MCK-Cre(+)双转基因小鼠与TβRⅡflox/flox转基因小鼠进行杂交,繁殖培育出在骨骼肌内特异敲除TβRⅡ基因的TβRⅡflox/flox/MCK-Cre(+)小鼠。结果:利用Cre/loxP技术世界上首次成功繁殖培育出有活力的且发育正常的TβRⅡ基因敲除小鼠。Objective： To generate the skeletal muscle-specific transforming growth factor beta receptor Ⅱ （TβRⅡ ）gene knockout mice for the research on the function of the TβRⅡ gene in skeletal muscles. Methods： TβRⅡ nox/noflox mice were generated using embryonic stem cell technology. The MCK-Cre mice were engineered containing Cre recombinase under the control of creatinkinase （MCK） muscle-specific promot- er. TβRⅡ flow/nox mice were crossed with MCK-Cre mice generating TβRⅡflox/wt/MCK-Cre double Tg mice. And then, TβRⅡ flox/wt/MCK- Cre（ ＋ ） double Tg mice were crossed withTβRⅡ nonox mice to generate TβRⅡ flow/wx/MCK- Cre（ ＋ ） mice genetically ablating TβRⅡ in cre-ex- pressing skeletal muscle cells. Results： As predicted, mice lacking TβRⅡ by gene targeting in skeletal muscles were generated first in the world using Cre/loxP system. TβRⅡ null mutant mice were viable, fertile and showed apparently normal development.

关 键 词：TβRⅡ 基因敲除 Cre/loxP技术 

分 类 号：Q812[生物学—生物工程]