木糖高效生物转化的新出路  

A New Way for Bioconversion of Xylose in High Efficiency

作　　者：徐勇[1,2,3] 王荥[1,2] 朱均均[1,2] 勇强[1,3] 余世袁[1,3] 

机构地区：[1]南京林业大学化工学院,南京210037 [2]江苏省生物质绿色燃料与化学品重点实验室,南京210037 [3]教育部林木遗传与生物技术省部共建重点实验室,南京210037

出　　处：《中国生物工程杂志》2012年第5期113-119,共7页China Biotechnology

基　　金：国家自然科学基金(31070514);江苏省科技支撑项目(BE2011838)资助项目

摘　　要：木糖的高效利用是影响木质纤维资源生物炼制经济效益的关键因素之一,也是构建其工业化生产体系的必要前提,但是木糖生物转化面临着重要的技术瓶颈,必须寻求新的思路。基于对木糖利用的现状及产业发展的综合分析,提出了木糖高效发酵制取木糖酸的新出路,论述了本领域首要的科学和技术问题是发酵抑制物的控制与消除;针对抑制物的问题,提出了细胞生理生化、代谢流分析及分子生物学的多层次和多尺度解析的研究方法;在此基础上,基于系统论的观点提出了菌种选育、原料预处理、抑制物控制与脱除、木糖酸高效发酵的技术集成的研究思路。Xylose utilization in high efficiency is a critical factor for the economic performance of lignocellulosic biomass biorefinry,and it is necessary for building of this industrial system.However,the novel ideas and thoughts for xylose utilization should be developed in that the bioconversion of xylose is now facing serious technical bottlenecks.Xylonic acid fermentation was brought forward as a new way for bioconversion of xylose from lignocellulosic biomass based on systemic analysis of its status and industry advancement.Controlling and elimination of inhibitors was illuminated as the primary problem of science and technology in xylonic acid fermentation,and then the approach to this challenge was put forward by a combined study of its cellular physiology and biochemistry,metabolism flux analysis and molecule biology with a multi-lever and multi-scale method.Furthermore,the tech-integration idea was brought up for industrial production of xylonic acid from xylose in a systemic view,involving development in steps of strains screening,material pretreatment,inhibitors controlling and removing and xylonic acid effective fermentation.

关 键 词：木糖的生物转化 木糖酸 木质纤维素资源 生物炼制 

分 类 号：Q81[生物学—生物工程]