生物催化中酰胺酶立体选择性的影响因素  

Factors Affect the Enantioselectivity of Amidase in Biocatalysis

作　　者：金建良[1] 徐建妙[1] 郑裕国[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《中国生物工程杂志》2012年第5期120-128,共9页China Biotechnology

摘　　要：立体选择性酰胺酶是一种重要的手性合成工具酶,在制备手性羧酸及其衍生物方面具有广阔的应用前景,日益受到重视。在酰胺酶的应用中,其立体选择性影响巨大。从底物、反应温度、pH、添加共溶剂和微生物来源5个方面综述了其对酰胺酶立体选择性的影响,对提高酰胺酶的立体选择性,扩大其在制备光学活性化合物领域的应用具有重要的意义。As a promising chiral synthesis tool,amidases have received growing attention with application in production of optically pure carboxyl acids and related derivatives.Enantioselectivity of amidase plays a vital role in amidase catalyzed reactions.Effects of microbial amidase sources and reaction factors such as substrate,temperature,pH as well as cosolvent on amidase enantioselectivity were summarized.It is helpful in improving amidase enantioselectivity and application of amidase in production of valuable optically pure compounds.

关 键 词：酰胺酶 立体选择性 影响因素 

分 类 号：Q55[生物学—生物化学]