喙端迁移流的发生及其细胞凋亡  

Development of the rostral migratory stream and its cell apoptosis

作　　者：厉永强[1] 商超蔚[1] 宋敏[1] 高文静[1] 石贞玉[1] 邓锦波[1] 

机构地区：[1]河南大学神经生物学研究所,河南开封475004

出　　处：《解剖学报》2012年第3期317-321,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30670688;30771140;31070952)

摘　　要：目的研究生后不同日龄小鼠喙端迁移流(RMS)的发育,神经干细胞增殖和凋亡的规律。方法利用Caspase-8免疫荧光标记法和5’-溴脱氧尿嘧啶核苷(BrdU)法,对小鼠RMS内的神经干细胞增殖和凋亡进行研究(n=92)。结果生后早期小鼠脑内,尤其是室管下区(SVZ)和RMS,存在大量的增殖细胞。随着小鼠年龄的增加,脑内干细胞逐渐减少,到成年,大脑皮质几乎见不到增殖的神经干细胞,但在SVZ和RMS仍可以看到许多增殖的神经干细胞。在RMS,神经干细胞增殖的同时伴随着细胞凋亡,干细胞的增殖与凋亡存在着正相关关系。结论 RMS的神经干细胞增殖与凋亡有重要的生理意义,通过细胞凋亡,RMS可以调节神经干细胞向嗅球迁移的数量,也可以调节干细胞向颗粒细胞分化。Objective To study the development of the rostral migratory stream （RMS） of mice and the proliferation and apoptosis of neural stem cells in RMS at various postnatal ages. Methods With 5-bromodeoxyuridine （BrdU） assay and Caspase-8 immunofluorescent staining, the neuroproliferation and cell apoptosis in RMS were investigated. Results In early postnatal phase, there were many proliferative eells in the brain, especially in subventricular zone （SVZ） and RMS. With age increasing, the stem cells in the brain decreased. In the adult, the stem cells in the neocortex were hardly found except in SVZ and RMS areas. When the proliferation of stem cells was companied with apoptosis during RMS development, there was a positive correlation between cell proliferation and apoptosis. Conclusion There is an important signification for the neuroproliferation and cell apoptosis in RMS. Through cell apoptosis, the migration and differentiation of neural stem cells in RMS are regulated.

关 键 词：神经干细胞 喙端迁移流 嗅球 免疫荧光 小鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]