肌肉特异性基因启动子的上游转录调控元件研究进展  

Progress of Upstream Transcriptional Control Elements in Promoters of Skeletal Specific Genes

作　　者：赵丹丹[1] 刘聪聪[1] 贾明玉[1] 杨悦[1] 叶枫[1] 严云勤[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《中国细胞生物学学报》2012年第5期500-505,共6页Chinese Journal of Cell Biology

基　　金：转基因生物新品种培育重大专项课题(No.2008ZX08007-002)资助项目~~

摘　　要：真核生物启动子位于基因5’端上游转录起始位点附近,是包含核心启动子以及上游转录调控元件的一段DNA序列,这些转录调控元件控制着基因表达的强度和特异性。肌肉特异性启动子的上游调控元件种类、数量和排列顺序决定着基因在肌肉中的特异性表达。深入研究肌肉启动子的上游调控元件,可以进一步了解肌肉基因表达机制,从而为肌肉性状的改良、增殖分化的机理和疾病的基因治疗等研究提供重要依据。该文回顾了近年来肌肉特异性启动子研究领域中的新发现,包括肌肉特异性启动子转录调控元件的分子机制、建立人工合成肌肉启动子的方法及应用,并探讨该领域中急需解决的问题和发展前景。The promoter of eukaryote, which is a short segment of DNA including core promoter and upstream transcriptional control element which regulates the strength and specificity of expression of genes, has been located at 5＇ upstream of a gene. The specificity of a muscle specific gene is determined by the variety, the quantity and the sequence of upstream control elements on muscle specific promoters. The further understanding of the mechanism of a gene expression would be gained by further studying upstream control elements of muscle promoters, and would lay a theoretical foundation for the study of the improvement of muscle traits, the mechanism of proliferation and differentiation, and the gene therapy of disease. In this paper, we review the new discoveries in the area of studying the artificial synthetic promoter, which includes the molecule mechanism of the transcriptional control element on the muscle specific promoter, and the method or application of establishing the artificial synthet- ic promoter, and we discuss the urgent problem and the development prospect which would be solved in this area.

关 键 词：肌肉特异性启动子 转录调控元件 人工合成启动子 

分 类 号：Q75[生物学—分子生物学]