程序性死亡因子1及其配体在糖尿病视网膜病变患者外周血单个核细胞中的表达  

The expression of programed death-1 and its ligands in peripheral blood mono-nuclear cells of diabetic retinopathy patients

作　　者：方梦园[1] 孟倩丽[1] 张良[1] 杨鑫[1] 朱杰宁[2] 郭海科[1] 

机构地区：[1]广东省人民医院眼科广东省眼病防治研究所广东省医学科学院,广州510080 [2]广东省人民医院眼科广东省眼病防治研究所广东省医学科学院医学研究中心,广州510080

出　　处：《中华眼底病杂志》2012年第3期228-231,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金（30901653）;广东省科技计划项目（2008B030301178,2008B030301170）;广东省医学科研基金（B2011013）

摘　　要：目的观察程序性死亡因子1（PD-1）及其配体PD-L1和PD-L2在糖尿病视网膜病变（DR）患者外周血单个核细胞（PBMCs）中的表达。方法临床检查确诊为DR的40例患者（DR组）以及同期行体检的健康志愿者20名（对照组）纳入研究。DR组40例患者中，非增生型DR（NPDR）20例，增生型DR（PDR）20例。抽取两组受检者晨起空腹静脉血2m1，采用荧光定量聚合酶链式反应（PCR）检测PD-1、PD-L1、PD-L2mRNA的表达水平。对比分析DR、对照组之间，PDR与NPDR组之间各指标表达水平的差异。结果荧光定量PCR检测发现，DR组患者PBMCs中PD-1、PD-L1mRNA相对表达量显著低于对照组，差异有统计学意义（t=-2．060，-2．562；P=0．043，0．013）；PD-L2mRNA相对表达量较对照组无明显变化，差异无统计学意义（t=-0．857，P=0．395）。PDR组患者PBMCs中PD-1tuRNA相对表达量较NPDR组有降低趋势，PD-I．1、PD-LI。2mRNA相对表达量有升高趋势，但差异均无统计学意义（t=-1．335，0．987，0．131；P：0．190，0．334，0．897）。结论DR患者PBMCs中PD-1、PD-L1tuRNA相对表达较正常者低，PD-L2mRNA相对表达较正常者无明显变化。Objective To observe the expression of programed death-1 （PD-1） and its ligands including PD-L1 and PD-L in peripheral blood mono-nuclear cells （PBMCs） of patients with diabetic retinopathy （DR） patients. Methods Forty patients with DR （DR group） and 20 healthy controls （control group） were included in this study. There were 20 patients with non-proliferative DR （NPDR） and 20 patients with proliferative DR （PDR）. Peripheral Blood samples were obtained from two groups. Real time polymerase chain reaction （RT-PCR） was used to analyze PD-1, PD-L1, and PD-L2 mRNA expression in PBMCs. The clinical data was analyzed in DR group and controls, also in PDR group and NPDR group. Results The results of RT-PCR showed that the expression of PD-1 and PD-L1 mRNA in DR group were significantly lower than those in the control group （t=-2. 060, -2. 562; P=0. 043, 0. 013）. There was no significant difference in PD-L2 mRNA expression between DR and control, group （t=- 0. 857, P= 0. 395）. Compared with the NPDR group, the lower expression level of PD-I mRNA and higher expression level of PD-L1 and PD-L2 mRNA in PDR group were observed, but the differences were not statistically significant （t=-1. 335, 0. 987, 0. 131; P=0. 190, 0. 334, 0. 897）. Conclusion PD-1 and PD-L1 mRNA expression in PBMCs of DR patients is decreased compared with controls, but there are no differences in PD- L2 mRNA expression in them.

关 键 词：糖尿病视网膜病变/病理生理学 基因表达 细胞死亡/生理学 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]