新型鸭呼肠孤病毒的分离与鉴定  

The Isolation and Identification of Novel Duck Reovirus

作　　者：陈少莺[1,2] 陈仕龙[1,2] 林锋强[1,2] 王劭[1,2] 江斌[1] 程晓霞[1,2] 朱小丽[1,2] 李兆龙[1,2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《病毒学报》2012年第3期224-230,共7页Chinese Journal of Virology

基　　金：国家863项目(2011AA10A209);福建省公益类项目(编号:2010R1025-5);国家自然基金(编号:31172334)

摘　　要：本研究从临床表现为出血性坏死性肝炎的病死鸭肝脾中分离到病毒。病原特性鉴定显示,分离毒能致死番鸭胚和鸡胚;人工感染1日龄雏番鸭、雏半番鸭均能复制出与临床自然发病鸭相同的临床症状和病理变化,并能回收到病毒。分离毒能在MDEF等多种细胞中增殖并产生细胞病变。电镜下病毒在细胞浆中呈大量散在、成堆和晶格状排列,病毒粒子呈球形、无囊膜、双层衣壳、直径70nm左右。在SDS-PAGE中具有禽呼肠孤病毒10个RNA片段的特征,但M1-3和S1-4片段的迁移率明显不同于番鸭呼肠孤病毒(MDRV)。分离毒S3基因全序列与禽呼肠孤病毒(ARV)、火鸡呼肠孤病毒(TRV)和MDRV的核苷酸同源性分别为60%～60.2%,61.9%,62.3%～62.7%,氨基酸同源性分别为68.2%～69%,68.2%,69.3%～70.1%;S3基因编码的σB蛋白属于单独的进化分支,提示分离毒S3基因具有不同于ARV和MDRV的特征。结果表明鸭出血性坏死性肝炎的病原是一种属于呼肠孤病毒科正呼肠孤病毒属新型鸭呼肠孤病毒。The virus strains were isolated from the liver and spleen of the dead young ducks characterized with symptoms of hemorrhagic-necrotic hepatitis. These isolates could cause the death of muscovy duck- embryo and chick-embryo. 1-day-old birds infected with these isolates had the same character with clinical- ly dead birds and the virus could be isolated from artificially infected birds. These isolates could proliferate in MDEF and result in CPE. The virus could proliferate in the cytoplasm in order of crystals and arranged in the latlic-like. The viron was shown spherical, icosahedron, cubic symmetry, no-envelope,with double- layered capsid, about 70nm in diameter by electron microscopy. The genome segments of the virus were consisted of L1-3, M1-3 and S1-4, which were similar to that of avian reovirus （ARV）. Compared to ARV, turkey reovirus （TRV） and muscovy duck reovirus （MDRV）, nucleotide homogy and acid amino homogy of S3 gene of the virus, were 60% -60.2%, 61.9%, 62.3% -62.7% and 68.2% - 69%, 68.2%, 69.3%-70.1%, respectively. The system evolution analysis showed that $3 gene coding B pro- tein was placed in different branch of MDRV and ARV, indicating thatS3 gene of the virus was different from ARV and MDRV. The main clinical symptoms and lesions of ducklings caused by the virus were dif- ferent from the diseases caused by MDRV and ARV. It was concluded that the virus was a Novel duck reo- virus belonging to Orthoreovirus genus of the Reoviridae family.

关 键 词：鸭 新型鸭呼肠孤病毒 出血性坏死性肝炎 分离 鉴定 

分 类 号：S853.65[农业科学—临床兽医学]