黑番鸭血管活性肠肽受体-1基因(VIPR-1)的cDNA克隆及其组织表达特性分析  

cDNA Cloning of Vasoactice Intestinal Peptide Receptor-1 Gene(VIPR-1) and Its Expression Characteristics in Tissues of Black Muscovy Duck(Cairina moschata)

作　　者：郑嫩珠[1] 陈晓燕[1] 陈晖[1] 朱志明[1] 缪中纬[1] 辛清武[1] 王正朝[2] 卢立志[2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《农业生物技术学报》2012年第5期529-535,共7页Journal of Agricultural Biotechnology

基　　金：福建省科技计划项目(No.2010R1025-6);国家现代农业产业技术体系建设专项(No.nycytx-45-06)

摘　　要：最近的研究表明，血管活性肠肽（VIP）与家禽的就巢习性密切相关。本研究采用反转录PCR方法从黑番鸭（Cairina moschata）母鸭下丘脑组织中克隆了血管活性肠肽受体（VIPR-1）基因的cDNA序列，长度为1125bp，编码355个氨基酸（GenBank登录号：JN625215）。序列比对结果表明，该序列与家禽（鸡、火鸡、鹌鹑）和哺乳动物（人、小鼠、猪、牛）的基因序列分别有93％-94％和69％-71％的同源性，而相应氨基酸序列的同源性分别为96％和70％-72％。荧光定量PCR结果发现，黑番鸭VIPR-1基因的表达量在产蛋期、就巢期和休产期差异显著（P〈0．05）或极显著（P〈0．01），就巢期表达量最高，休产期次之，产蛋期表达量最低，表明VIPR-1基因与繁殖阶段变化密切相关；对不同组织VIPR-1的表达量分析发现，VIPR-1在垂体、下丘脑和卵巢中均有表达，其中垂体最多，其次是下丘脑，卵巢中的表达量最低，差异极显著（P〈O．01）。研究结果提示，VIPR-1基因具有高度保守性，参与下丘脑-垂体-卵巢轴（特别是垂体）对黑番鸭就巢行为的调控。Recent studies have already indicated that vasoactice intestinal peptide （VIP） is closely related to the habit of birds＇ nesting. In this study, we first cloned VIP receptor gene and analyzed its characteristics of expression in different tissues of muscovy duck. Reversed transcript PCR was used to clone vasoactice intestinal peptide receptor-1 （VIPR-1） gene from the hypothalamus of black muscovy ducks（Cairina moschata）, the cDNA sequence of this gene consisted of 1 125 nucleotides, which coded 335 amino acids （GenBank accession No. JN625215）. The sequence of VIPR-1 was highly conserved in avian species. The muscovy duckReal-time PCR, and the highest expression level was in the stage of incubating, while the lowest was in out-of-lay. This finding indicated that expression level V1PR-1 was closely correlated with the reproduction stages. In addition, VIPR-I mRNA was found to be expressed in the hypothalamus, pituitary and ovary of black muscovy ducks. The highest expression level of VIPR-1 was found in hypothalamus, while the lowest level was in ovaries （P〈0.01）. In summary, VIPR-1 gene is highly conservative, and its tissue-specific expression patterns imply that VIPR-1 gene is involved in the role of hypothalamus-pituitary-ovary axis in regulating the incubation of muscovy ducks.

关 键 词：黑番鸭 血管活性肠肽受体基因(VIPR-1) 就巢 实时荧光定量PCR 

分 类 号：Q516[生物学—生物化学]