卵菌基因功能分析方法的研究进展  

Advances on the Methods Used for the Functional Analysis of Oomycete Genes

作　　者：陈孝仁[1] 王源超[2] 

机构地区：[1]扬州大学园艺与植物保护学院,扬州225009 [2]南京农业大学植物保护学院,南京210095

出　　处：《农业生物技术学报》2012年第5期568-575,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金资助项目(No.31101395);江苏省基础研究计划(自然科学基金)资助项目(No.BK2011443);扬州大学高层次人才科研启动资助项目

摘　　要：卵菌是色菌界(Chromista)中一类二倍体真核微生物,包括许多重要的植物病原菌。由于缺乏有效的遗传和分子生物学工具,在分子水平上的研究进展一直比较缓慢。为促进人们对卵菌分子遗传学的了解,探索成熟的基因功能分析方法,本文综述了目前卵菌基因功能研究中使用的三类分析方法:干扰基因表达的方法、缺失特定基因的方法和促使基因表达的方法。重点论述了干扰基因表达进行卵菌基因功能分析的方法,讨论了三类方法中不同技术的优缺点,并对未来的发展方向进行了展望。Oomycetes is a class of diploid eukaryotic microorganisms in the kingdom of Chromista, including many of important plant pathogens. The principal impediment to progress in the molecular analysis of oomycetes has been the lack of efficient genetic and molecular biological tools. To evoke researchers to better understand the molecular genetics of oomycets and find out efficient tools for functional analysis of genes, three kinds of methods （disturbing gene expression, disrupting specific genes and spurring gene expression） used for the gene function analysis in oomycete study are reviewed in this paper. The review places an empha- sis on the kind of disturbing gene expression. The advantages and disadvantages of the different techniques in three kinds were discussed. Future research trends of molecular genetics tools were discussed as well.

关 键 词：卵菌 遗传转化 基因沉默 RNA干扰 农杆菌感染法 

分 类 号：Q78[生物学—分子生物学]