聚铃花的组织培养与无性系的建立  

Tissue Culture and Asexual Line Establishment of Campanula glomerata

作　　者：滕贺[1] 孙铭鸿[1] 马楠[1] 邹翠霞[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《贵州农业科学》2012年第4期24-26,共3页Guizhou Agricultural Sciences

基　　金：辽宁省高等教育教学改革资助项目"开放实验室--植物克隆技术研究实践的人才培养模式改革与创新"(20090304);辽宁师范大学教学改革资助项目"开放实验室本科生创新能力和创新思维培养的研究"(LSJG:20090108)

摘　　要：为获得大量种苗,满足观赏栽培的需求,采用组织培养建立无性系的方法对聚铃花进行了研究。结果表明:MS+0.6mg/L 6-BA+0.3mg/L NAA是带叶茎段生长分化培养的理想培养基;White+1.5mg/L GA3+0.1mg/L IAA是分化不定芽生长培养的理想培养基;生长芽下部剪口在10mg/L NAA的无菌溶液中处理5min后,接种到1/3MS+0.1mg/L IAA+0.2mg/L NAA培养基上是聚铃花生长芽生根培养的理想方法;试管苗移栽定植易成活,定植的试管苗生长正常,观赏效果好。In order to meet the need for cultivation of germchits,the tender stems with leaves of C.glomerata were used as materials to do the research by using tissue culture methods.The results showed that MS＋6-BA 0.6 mg/L ＋ NAA 0.3 mg/L was the optimum medium for differentiation of stems with leaves.The optimum medium for adventitious buds growth was White＋GA31.5 mg/L＋IAA 0.1 mg/L.After dealt with NAA（concentration of 10 mg/L） for five minutes,the adventitious buds could be easily induced rooting in the medium of 1/3 MS＋IAA 0.1 mg/L＋NAA 0.2 mg/L.The tube seedlings could be easy to survive after transplanting.Colonization of plantlets grew normally and had good view and admire.

关 键 词：聚铃花 愈伤组织 无性系 快速繁殖 

分 类 号：S567.23[农业科学—中草药栽培]