大豆籽粒不同发育时期基因表达谱分析  

Gene expression profile of developing soybean seeds

作　　者：陈欢[1,2] 杨美英[1] 王真慧[3] 孙旸[2] 王刚[2] 魏玮[2] 关瑜[2] 王法微[1] 李海燕[1,2] 陈光[2] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118 [3]东北师范大学分子表观遗传学教育部重点实验室,吉林长春130024

出　　处：《中国油料作物学报》2012年第2期129-135,共7页Chinese Journal of Oil Crop Sciences

基　　金：科技部863项目(2011AA1000691008);国家转基因生物新品种培育重大专项(2010ZX08010-002);吉林省优秀创新团队项目(20111815)

摘　　要：以花后15d的大豆籽粒作为对照,通过Solexa高通量测序方法对花后35d、55d和65d的大豆籽粒进行转录水平上的检测,并结合GO功能注释和pathway分析,共有差异表达unigene为9 905个,其中调控脂肪酸合成途径的显著差异表达基因12个,蛋氨酸代谢途径关键基因6个。以花后55d的cDNA为样本,荧光定量PCR检测了12个差异表达基因,其结果可与测序结果互为印证。Solexa high-throughput RNA-sequencing were performed on 35DAF（days after flowering）,55DAF and 65DAF samples at transcriptional level,while seeds at 15DAF served as control.Totally 9 905 differential expressed unigenes were co-expressed genes.Combined with GO functional annotation and pathway analysis,12 novel co-expressed unigenes that regulated fatty acid biosynthesis pathway and 6 novel co-expressed unigenes that regulated methionine metabolism were found.qRT-PCR was focused on 12 differentially expressed genes from cDNA materials of 55DAF.Results were consistent with those from Solexa sequencing.

关 键 词：大豆 油脂 蛋白质 脂肪酸 蛋氨酸 差异表达基因 

分 类 号：S565.103[农业科学—作物学]