富含硫氨基酸转基因大豆对根际土壤微生物群落结构的影响  

Effect of transgenic soybean with sulfur-rich amino acids on soil microbial population structure

作　　者：桂恒[1] 张培培[1] 华小梅[2] 彭欣[1] 孔令如[1] 陈丰[1] 戚金亮[1] 喻德跃[3] 杨永华[1] 

机构地区：[1]南京大学生命科学学院,医药生物技术国家重点实验室,南京大学-南京林业大学植物分子生物学联合研究所,江苏南京210093 [2]环境保护部南京环境科学研究所,江苏南京210042 [3]南京农业大学国家大豆改良中心,作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《中国油料作物学报》2012年第2期181-187,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家重大专项(2011ZX08011-003);国家自然科学基金(30971871,40771107);教育部创新团队项目(IRT1020);教育部高校科技创新工程重大项目培育资金(707027)

摘　　要：选用两组转基因品质改良大豆(富含硫氨基酸),利用磷脂脂肪酸(PLFA)法分析了在大田种植条件下,两组转基因大豆对土壤微生物群落结构的影响。结果表明,与非转基因大豆相比,转基因品系根际土壤微生物群落的结构发生明显的变化。在两组转基因品系中PLFA组分均发生变化,A组中检测到15种特异性的PLFA,其中占主导地位的分别是是革兰氏阳性菌(GP)的特征脂肪酸16∶0、10Me18∶0和好氧细菌特征脂肪酸18∶1ω7c;B组中检测到13种特异性的PLFA,其中占主导地位的是表征GP的脂肪酸15∶0、16∶0和非特征脂肪酸14Me16∶0。在A组的OE-8品系及B组的17-4和57品系中,表征细菌的PLFA含量和总PLFA含量与非转基因品系相比有显著上升。结果表明种植转基因大豆影响根际土壤微生物群落结构。Effects of transgenic soybeans on microbial population structure of rhizosphere soil were investigated under field conditions based on phospholipid fatty acid（PLFA） method.Two groups of transgenic soybeans with sulfur-rich amino acids were used.Results showed that soil microbial population in rhizosphere of transgenic lines changed significantly in both group A and B.15 specific PLFAs were identified in group A,and 13 in group B.Correlation analysis found that microbial characterization of group A and the total concentration of PLFA＇s of the transgenic line OE-8 were significantly higher than non-transgenic line,and so were 17-4 line and 57 line in group B.The dominant fifteen PLFA mown plots were 16∶ 0,10Me18∶ 0 and 18∶ 1ω7c in group A,and dominant thirteen PLFA were 15∶ 0,16∶ 0 and 14Me16∶ 0 in group B.The results suggested that the microbial population structure changed after planting transgenic soybeans.

关 键 词：转基因大豆 土壤微生物群落 磷脂脂肪酸 

分 类 号：S565.1[农业科学—作物学] S154.36