快速检测痕量铜的光电型传感器的研制  

Research of Photoelectric Sensor for Fast Test of Trace Copper

作　　者：商璟[1] 柴春彦[1] 翁芝莹[1] 张洪才[1] 刘国艳[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《环境监测管理与技术》2012年第2期45-47,50,共4页The Administration and Technique of Environmental Monitoring

基　　金：上海市科委技术标准专项基金资助项目(06DZ05036)

摘　　要：利用铜对葡萄糖氧化酶的抑制作用,以及葡萄糖氧化酶-辣根过氧化物酶-葡萄糖(Glu)-邻联甲苯胺(OT)偶联反应体系生成蓝色底物的原理,将Glu与OT固定化制成试纸条,插入自制的便携式单色光反射计,组装出检测铜的光电型传感器。当铜的质量浓度范围为1 mg/L～100 mg/L时,光反射强度值与铜的质量浓度呈良好的线性关系。在优化的试验条件下,该方法的检测限为0.064 mg/L,检测时间为5 min。A photoelectric sensor was composed by home-made portable monochromatic light reflector which inserted a test strip with immobilized glucose and o-tolidine for copper detection.Principle of the photoelectric sensor was that inhibitory effect of copper on glucose oxidase formed blue substrate by using a coupling reaction system of glucose oxidase-horseradish peroxidase-glucose-o-tolidine.There was good correlative linearity between 1 mg/L^100 mg/L copper solution and light reflecting intensity.The detection limit of method was 0.064 mg/L,testing time 5 minutes after optimization of the experimental condition.

关 键 词：铜 光电型传感器 葡萄糖氧化酶-辣根过氧化物酶-葡萄糖-邻联甲苯胺 快速检测 

分 类 号：O657.3[理学—分析化学]