重型再生障碍性贫血患者CD8＋HLA—DR＋T淋巴细胞效应因子mRNA的表达  

Expression of lymphokines in CD8 ＋ HLA-DR ＋ T lymphocytes of patients with severe aplastic anemia

作　　者：刘春燕[1] 付蓉[1] 王珺[1] 梁勇[1] 瞿文[1] 阮二宝[1] 王化泉[1] 李丽娟[1] 刘惠[1] 王国锦[1] 刘鸿[1] 吴玉红[1] 王晓明[1] 宋嘉[1] 邢莉民[1] 关晶[1] 王红蕾[1] 张田[1] 刘晓[1] 邵宗鸿[1] 

机构地区：[1]天津医科大学总医院血液肿瘤科,300052

出　　处：《中华医学杂志》2012年第18期1240-1243,共4页National Medical Journal of China

基　　金：国家自然科学基金（30971286、30971285）;卫生部卫生行业科研专项（201002024）;高等学校博士学科点专项科研基金（200800620004）;天津市应用基础及前沿技术研究计划（08JCYBJC07800、09JCYBJC11200）;天津市卫生局科技攻关项目（11KG135）

摘　　要：目的观察重型再生障碍性贫血（SAA）患者外周血CD8＋HLA—DR＋T淋巴细胞效应因子水平，进一步探讨SAA的免疫发病机制。方法采用免疫磁珠分选24例SAA初治患者及23名健康对照外周血CD8＋HIJA-DR＋效应T细胞，半定量反转录PCR检测分选细胞穿孔素、颗粒酶B、Fas配体（FasL）、肿瘤坏死因子β（TNF-β）mRNA表达情况。结果SAA初治组CD8＋HLA－DR＋效应T细胞的穿孔素、颗粒酶B、FasL、TNF－βmRNA的表达量分别为0．66±0．25、0．56±0．26、0．77±0．24、0．58±0．16，均明显高于健康对照（0．53±0．14、0．40±0．13、0．61±0．16、0．46±0．15，P=0．042、0．012、0．011、0．011）。结论CD8＋HIA—DR＋效应T细胞可能通过穿孔素、颗粒酶B途径、Fas／FasL途径及TNF-B途径损伤SAA患者骨髓造血细胞，在SAA免疫发病过程中发挥了重要作用。Objective To examine the expression of lymphokines damaging hematopoietic cells by CD8 ＋ HLA-DR ＋ effector T cells in peripheral blood （PB） of the patients with severe aplastic anemia （SAA） and explore further the heterogeneous immunopathogenesis of SAA. Methods The CD8 ＋ HLA-DR ＋ cells were sorted by immunomagnetic separation from the PB of 24 untreated SAA patients and 23 normal controls. The mRNA expressions of perforin, granzyme B, FasL and tumor necrosis factor I＋ （TNF-13） of sorted cells were analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. Results The mRNA levels of perforin, granzyme B of CD8 ＋ HLA-DR ＊ T cells in untreated group were higher than those of the controls （0. 66 ± 0. 25,0. 56 ± 0. 26 vs 0. 53 ± 0. 14, 0. 40 ± 0. 13,P = 0. 042, 0. 012）. The mRNA level of FasL in CD8 ＋ HLA-DR＋ T cells of untreated SAA patients was higher than that of the controls （0. 77 ＋ 0. 24 vs 0. 61 ＋0. 16, P =0. 011 ）. The mRNA of TNF-[3 in CD8 ＋ HLA-DR＋ T cells of untreated SAA patients was also higher than that of the controls （ 0. 58 ± 0. 16 vs 0.46 ± 0. 15, P = 0.011 ）. Conclusions CD8 ＋ HLA- DR ＋ T cells may damage hematopoiesis through the actions of perforin, granzyme B, TNF-β and FasL And it thus contributes to the immunopathogenesis of SAA.

关 键 词：贫血 再生障碍性 颗粒酶类 肿瘤坏死因子类 穿孔素 FAS配体 

分 类 号：R556[医药卫生—血液循环系统疾病]