角膜上皮细胞体外培养技术的研究进展  

Research advances on in vitro culture techniques of corneal epithelial cells

作　　者：王瑞鑫[1] 樊廷俊[1] 

机构地区：[1]中国海洋大学海洋生命学院角膜组织工程实验室,山东青岛266003

出　　处：《山东大学学报（医学版）》2012年第6期31-37,共7页Journal of Shandong University:Health Sciences

基　　金：国家高技术研究发展863计划(2006AA02A132)

摘　　要：体外重建组织工程角膜并将其用于角膜移植是使角膜盲患者复明的惟一有效途径,其中角膜上皮种子细胞的来源成为急需解决的关键问题。由于体外生长的角膜上皮细胞生命周期短,因此通过改进体外培养技术来获得增殖能力强的细胞成为解决角膜上皮种子细胞来源问题的首要任务。本研究从角膜上皮细胞的体外培养技术包括培养方法和培养条件这两方面进行了综述。In vitro reconstruction of tissue-engineered corneas for transplantation is an effective way to cure corneal blindness, and the key issue is the source of corneal epithelial seed cells. Due to the short life-span of corneal epithelial ceils in vitro, improvement of culture techniques to obtain the ceils with strong proliferation ability is believed to solve the seed cells source problem. In this present paper, we reviewed the in vitro culture techniques of corneal epithelial ceils including culture methods and conditions.

关 键 词：组织工程角膜 角膜上皮细胞 种子细胞 体外培养 

分 类 号：R318[医药卫生—生物医学工程]