注射硫代乙酰胺并饲喂酵母培养物、姜黄素和水飞蓟素对草鱼脂代谢相关基因表达丰度的影响  

Effects of yeast culture,curcumin and silymarino on lipid metabolismrelated genes expression activity of liver injured by thioacetamide in grass carp

作　　者：许凡[1] 叶元土[1] 蔡春芳[1] 张宝彤[2] 萧培珍[2] 王丽宏[2] 向朝林[1] 姚林杰[1] 刘猛[1] 李薇[3] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏省水产动物营养重点实验室,江苏苏州215123 [2]北京市营养源研究所系统营养工程技术研究中心,水产动物系统营养研究开放实验室,北京100069 [3]中山大学生命科学学院,广东广州510275

出　　处：《水产学报》2012年第5期740-747,共8页Journal of Fisheries of China

基　　金：苏州市应用基础(农业)项目(SYN201003)

摘　　要：为探讨硫代乙酰胺(TAA)诱导草鱼肝胰脏损伤与损伤修复的作用机制,试验以腹腔注射硫代乙酰胺诱导肝胰脏损伤实验模型草鱼为对象,分别饲喂含有酵母培养物dv、姜黄素和水飞蓟素的饲料70 d后,采集草鱼肝胰脏样品,采用实时定量反转录聚合酶链式反应(qPCR)方法,检测了草鱼肝胰脏脂肪酸合成酶(FAS)、过氧化物酶增殖体激活受体γ辅助激活因子(PGC1-α)、硬脂酰辅酶A去饱和酶1(SCD1)、解偶联蛋白2(UCP2)、过氧化物酶增殖体激活受体α(PPAR-α)、过氧化物酶增殖体激活受体γ(PPAR-γ)、类胰岛素样生长因子(IGF-Ⅰ)的mRNA丰度,探讨在饲料中添加酵母培养物dv、姜黄素和水飞蓟素后,草鱼肝胰脏脂代谢相关基因表达丰度的变化。结果显示,TAA诱导草鱼肝损伤后,FAS基因表达丰度上调,调控脂质分解代谢的上游基因(如PPAR-α、ΡΡΑR-γ、PGC1-α)表达丰度没有显著性的变化,而SCD1基因表达丰度显著性下调,其结果可能导致脂质在肝细胞积累量增加,诱发脂肪性肝病的发生和发展。在饲料中添加酵母培养物dv、姜黄素和水飞蓟素后,在一定程度上修复了TAA对肝胰脏脂代谢的损伤。To discuss and study the damage and repair mechanism of the thioacetamide-induced fish liver lesions in our research,firstly we established a liver injury experimental model of grass carp by intraperitoneal injection of thioacetamide（TAA）.Then fish were fed with additive yeast culture dv,curcumin and silymarin for 70 days,and the hepatopancreas samples were collected.We used real-time quantitative reverse transcription polymerase chain reaction（qPCR） method to detect the mRNA abundance of FAS,PGC1-α,SCD1,UCP2,PPAR-α,PPAR-γ and IGF-Ⅰso as to discuss the changes of the expression activity of the lipid metabolism-related genes of hepatopancreas of fishes in the experimental models as well as that which were fed with the three damage-repair additives such as yeast culture dv,curcumin and silymarin.The results showed that,after the thioacetamide induced liver injury,the expression level of FAS was significantly increased,the upstream genes regulating the lipid catabolism such as PPAR-α,ΡΡΑR-γ and PGC1-α had no significant change,while the SCD1 was significantly reduced,which may result in the increased lipid accumulation in the liver cells,so as to induce the occurrence and development of fatty liver disease.In brief,after feeding additives such as yeast culture dv,curcumin and silymarin,thioacetamide induced damage of the lipid metabolism of the grass carp hepatopancreas was to some extent repaired.

关 键 词：草鱼 损伤 脂代谢 表达丰度 

分 类 号：Q785[生物学—分子生物学] S963.73[农业科学—水产养殖]