白藜芦醇对黑色素瘤细胞增殖的抑制作用  

Inhibition of resveratrol on the proliferation of melanoma cells

作　　者：孟德萍[1] 邹志孟 许晓群[1] 张建华[1] 姜国胜[1] 王郡甫[1] 

机构地区：[1]山东省医学科学院基础医学研究所山东省罕少见病重点实验室,山东济南250062 [2]烟台市牟平区人民医院放射科,山东烟台264100

出　　处：《中华肿瘤防治杂志》2012年第5期328-331,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省自然科学基金(ZR2009CM036)

摘　　要：目的:探讨白藜芦醇对黑色素瘤细胞WM239增殖、细胞周期及凋亡的影响。方法:MTT法检测白藜芦醇对WM239细胞增殖的影响;流式细胞术检测白藜芦醇对WM239细胞周期及凋亡的影响。结果:12.5～200μmol/L的白藜芦醇作用于WM239细胞48h和72h后,对其增殖具有一定的抑制作用,作用72h时,浓度>50μmol/L其抑制率>50%,且抑制程度与药物浓度呈正相关。其中0、25、50和100μmol/L药物与WM239细胞作用48h后,凋亡率分别为0.1%、9.7%、8.9%和19.3%,处理组与未处理组相比细胞凋亡明显增加,G0/G1期细胞分别为71.9%、77.3%、81.0%和83.7%,S期分别为21.5%、17.9%、16.0%和14.6%,G2/M期为6.5%、4.8%、2.9%和1.8%,细胞周期阻滞于G1期,S期和G2期细胞比例下降,剂量依赖关系明显。结论:白藜芦醇可以抑制WM239细胞的增殖,引起细胞周期改变,凋亡细胞明显增加。OBJECTIVE： To explore the influence of resveratrol on the growth,cell cycle and apoptosis of melanoma cell lines WM239. METHODS： The effects of resveratrol on the proliferation of WM239 cells were analyzed with MTT as- say. The cell cycle and apoptosis was analyzed using flow cytometry with Annexin-V and PI staining. RESULTS： The proliferation of WM239 was inhibited when the cell line was exposed to 12.5--200μmol/L resveratrol for 48 h and 72 h. The inhibition rate was higher than 50% when the cell line was exposed to above 50 vmol/L resveratrol for 72 h. The inhibition was positively correlated to drug concentration. Exposed to 0,25,50 and 100/Lmol/L resveratrol for 48 h, the apoptosis rate were 0.1%, 9.7%, 8.9% and 19.3%. G0/G1 phase cells were 71.9%, 77.3%, 81.0% and 83.7% respectively. S phase cells were 21.5%, 17.9%, 16.0% and 14.6%. G2/M phase cells were 6.5%, 4.8%, 2.9% and 1.8%. The cell cycle was blocked in G1 phase. The cells of S and G2 phase decreased in a dose-dependent manner. CONCLU- SION：Resveratrol could inhibit the proliferation of WM239 cells, affect the cell cycle and cause the apoptosis of WM239 cells.

关 键 词：白藜芦醇 黑色素瘤细胞 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]