弱激光照射对实验性牙移动牙周组织血管内皮细胞中整合素αVβ3表达的影响  

The Effect of Lower Power Laser Irradiation on the Expression of Integrin αVβ3 in Periodontium during Experimental Tooth Movement

作　　者：孙秀梅[1] 孙新华[1] 吴国民[1] 张晓英[1] 

机构地区：[1]吉林大学口腔医学院正畸科,吉林长春130021

出　　处：《口腔医学研究》2012年第5期409-412,共4页Journal of Oral Science Research

摘　　要：目的:探讨弱激光照射对兔实验性牙移动牙周组织整合素αVβ3表达的影响。方法:35只大耳白兔随机分为7组,每组5只,分别作为对照组,1、3、5、7、14、21d组。左侧为对照侧,右侧为弱激光照射侧。于上颌第一磨牙与同侧切牙间置不锈钢螺簧,以80g力拉磨牙向近中。按期分批处死动物,取标本,通过原位杂交方法检测牙周组织中整合素αVβ3mRNA的表达。结果:经弱激光照射后,照射侧牙周组织血管内皮细胞中整合素αVβ3表达高于对照侧。照射侧压力区血管内皮细胞中整合素αVβ3表达在1d时明显低于对照侧,3、5、7d时明显高于对照侧;照射侧张力区整合素αVβ3表达在1、3、5、7d时显著高于对照侧。结论:弱激光照射促进了牙周组织血管内皮细胞中中整合素αVβ3的表达,这可能是其促进牙周组织改建,减轻正畸疼痛的机制之一。Objective： To investigate the effect of low power laser on integrin αVβ3 expression in periodontal tissue during orthodontic tooth movement. Methods： Thirty--five rabbits were divided into 7 groups on average： untreated （control）, ld, 3d, 5d, 7d, 14d, and 21d group. Except the control group, the right cheek of the other rabbits re- ceived irradiation of lower power laser as the experimental side and the left side was considered as the control side. A elastic coil spring was ligated between the incisors and the first molar and exert a force of approximately 80 g to pull the first molar mesially. The animals were killed on time respectively and specimens were made to observe the expression of integrinαVβ3 in periodontium through in situ hybridization. Results： The expression of integrinβ3 in the vascular endothelial cells in the compression region of the irradiated side was apparently less than the control side on the first day and was apparently more than the control side on the third day and the fifth day. Differently, the ex- pression of integrinβ3 in the tesion region of the irradiated side was apparently more than the control side from the first day to the seventh day. Conclusion.- The irradiation of lower power laser can effectively promote integrin αVβ3 expression,by which the lower power laser benefit the remodeling of the periodontium.

关 键 词：牙齿移动 血管形成 整合素ΑVΒ3 原位杂交 

分 类 号：R783.5[医药卫生—口腔医学]