斑秃患者肿瘤坏死因子α-238G/A和-308G/A基因多态性的研究  

Study on the polymorphism of TNFα-238G/A and -308G/A gene in patients with alopecia areata

作　　者：谭凤明[1] 姚海峰[1] 卢浩锵[1] 程喜平[1] 侯显曾[1] 陈盛强[2] 

机构地区：[1]广州医学院第一附属医院皮肤科,广东广州510120 [2]广州医学院第二附属医院神经科学研究所,广东广州510260

出　　处：《临床皮肤科杂志》2012年第6期342-345,共4页Journal of Clinical Dermatology

基　　金：广东省科技厅自然科学基金(2010B031600303)资助项目

摘　　要：目的:探讨肿瘤坏死因子(TNF)-α的-238G/A和-308G/A基因多态性与斑秃的关系。方法:运用多聚酶链反应技术检测102例斑秃及141例正常人TNF-α的-238G/A和-308G/A基因多态性。结果:斑秃组-238 G/G、G/A和A/A基因型频率分别为0.9510、0.490和0,对照组分别为0.9574、0.0426和0;斑秃组-238G和-238A基因频率分别为0.9755和0.0245,对照组分别为0.9787和0.0213。-238C/A各种基因型频率在患者组与正常对照组之间的差异无统计学意义(P>0.05)。斑秃组-308C/C、G/A和A/A基因型频率分别为0.9118、0.0784和0.0098,对照组分别为0.9277、0.0567和0.0213;斑秃组-308G和-308A基因频率分别为0.9510和0.0490,对照组分别为0.9504和0.0496。斑秃组TNF-α-238G/A和-308G/A两位点单体型分析结果表明,这两个位点组成的单体型不存在过度传递。结论:TNF-α的-238G/A和-308G/A基因多态性与斑秃可能没有相关性。Objective： To investigate the polymorphism of TNFα-238G/A and -308 G/A gene in alopecia areata. Methods： The polymorphisms of TNFα-238G/A and TNFα-308 G/A genes were screened by PCR-RELP. Results： In AA group the genotype frequency of TNFα-238G/G, G/A, A/A, 238G and 238A was 0.951, 0.490, 0, 0.9755 and 0.0245 respectively. But in normal control, it was 0.9574, 0.0426, 0, 0.9787 and 0.0213 respectively. There was no difference between the two groups （P〉0.05）. And also, for the genotype frequency of TNFα-308 G/G, G/A, A/A, -308G and -308A, it was 0.9118, 0.0784, 0.0098, 0.9510 and 0.0490 respectively in AA group and 0.9277, 0.0567, 02130, 0.9504 and 0.0496 in normal control. There was also no statistic difference between the two group （P 〉 0.05）. Conclusion： The results suggest that there is no association of alopecia areata with the polymorphisms of NFα-238G/A and TNFα-308G/A.

关 键 词：斑秃 TNF-α-308G/A TNF-α-238G/A 多态性 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]