微柱凝胶法ABO血型鉴定正反定型不相符的统计及原因探讨  

Statistics and causes analysis on unconformity of positive and negative determining results of ABO blood group identification by micro-column gel agglutination assay

作　　者：张海宇[1] 张珂[1] 

机构地区：[1]四川省肿瘤医院输血科,成都610041

出　　处：《检验医学与临床》2012年第11期1295-1296,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨分析本院10 136例肿瘤患者微柱凝胶法ABO血型鉴定中正反定型结果不符的比例及原因,使交叉配血实验顺利进行,保证临床用血的安全有效。方法对本院2007年1月至2010年12月肿瘤患者做微柱凝胶法ABO血型鉴定中正反定型结果不符进行统计及原因分析。结果在本院10 136例肿瘤患者微柱凝胶法ABO血型鉴定中,正反定型结果不符的28例,占总数的0.28%。在这28例的标本中,肿瘤患者抗体减弱15例,占53.6%;冷凝集素8例,占28.6%;ABO血型亚型2例,占7.1%;人为技术错误2例,占7.1%;多发性骨髓瘤1例,占3.6%。结论肿瘤患者微柱凝胶法ABO血型鉴定中正反定型结果不相符中,肿瘤患者抗体减弱占据首位,其次是冷凝集素,所以检验人员必须严格遵守操作规程,熟练各种试验技术,认真对待每一份待检标本,辨析各种异常情况,掌握鉴定方法,确保患者用血安全。Objective To investigate the proportion of unconformity of positive and negative determining results of ABO group identification by micro-column gel agglutination assay （MGAA） in 10 136 tumor patients and to analyze their reasons for conducting blood cross matching smoothly and ensuring the safety and effect of clinical blood use. Methods The unconformity of positive and negative determining results of ABO blood group identification by MGAA in tumor patients from January 2007 to December 2010 was statistically performed the reason analysis. Results In 10 136 cases of ABO blood group identification by MGAA,28 cases were unconformity in positive and negative determining results, accounting for 0.28% of total number. Among these 28 specimens, 15 cases （53.6%） were antibody decrease, 8 cases（28.6 % ） were cold agglutinin, 2 cases （7.1%） were ABO group subtypes, 2 cases （7.1% ） were human failures and l case（3.6%） was multiple myeloma. Conclusion Among unconformity cases of positive and negative determining results of ABO blood group identification by MGAA, the top place is antibody decrease, and followed by cold agglutinin. Therefore the laboratory personnel must strictly abide the operation regulation, proficiently master various test techniques, treat each testing specimen seriously, discriminate various abnormal situations and master identification methods for ensuring the patientr s safety of blood use.

关 键 词：微柱凝胶法 ABO血型 正反定型 

分 类 号：R457.11[医药卫生—治疗学]