程序化细胞死亡因子5在支气管哮喘患者血清中的表达及其意义  

Expression and significance of programmed cell death 5 in patients of branchial asthma

作　　者：刁筱琳[1] 朱红[1] 贺蓓[1] 潘欢[2] 伍蕊[1] 盖晓燕[1] 

机构地区：[1]北京大学第三医院呼吸内科,100191 [2]北京大学人类疾病基因研究中心

出　　处：《中华医学杂志》2012年第20期1392-1395,共4页National Medical Journal of China

基　　金：国家自然科学基金（30770939）

摘　　要：目的探讨程序化细胞死亡因子5（PDCD5）在支气管哮喘患者血清中的表达及其意义。方法将2011年6—12月北京大学第三医院呼吸科门诊及病房诊治的40例成人支气管哮喘患者作为哮喘组（其中急性发作期12例，慢性持续期14例，临床缓解期14例），15名同期在体检中心进行健康体检者作为对照组。收集并检测两组受试者外周血中性粒细胞、嗜酸粒细胞百分数，酶联免疫吸附法测定血清PDCD5水平，填写哮喘控制测试（ACT）问卷，使用肺功能仪测定肺功能相关指标；对检测结果进行统计学分析。结果哮喘患者外周血中性粒细胞及嗜酸粒细胞百分数与对照组比较，差异均无统计学意义（均P〉0．05），哮喘组血清PDCD5表达量显著高于对照组[（47．7±29．6）比（19．3±9．8）μg／L，P〈0．05]，急性发作期、慢性持续期患者血清PDCD5显著高于临床缓解期[（55．2±24．5）、（68．5±22．1）比（16．0±7．9）μg／L，均P〈0．05]，哮喘患者血清PDCD5表达量与第1秒用力呼气量（FEV1）占预计值的百分比（FEV1％）、FEV1与用力肺活量的比值（FEV，／FVC）及ACT问卷评分呈负相关（r=-0．539～-0．798，均P〈0．05）。结论PDCD5参与了哮喘的气道炎症过程，其表达异常可能与哮喘未控制有关，可能成为临床上协助评估哮喘控制情况的指标或治疗的靶点。Objective To explore the expression and significance of serum programmed cell death 5 （ PDCD5 ） in patients with bronchial asthma. Methods From June to December 2011, a total of 40 adults with bronchial asthma treated in Peking University Third Hospital were enrolled. Among them, the categories were acute phase （n = 12）, chronic phase （n = 14） and remission phase （n = 14）. Fifteen healthy adults were selected into the control group. The percentages of peripheral blood neutrophils and eosionphils were collected and detected for each patient. Serum PDCD5 was detected with enzyme-linked immunosorbent assay （ELISA） and asthma control test （ACT） questionnaire filled in. The relevant pulmonary functional indicators were analyzed with a pulmonary spirometer. Two-independent sample t-test and Pearson＇s correlation analysis were used for statistical analysis. Results No significant difference was found between two groups with regards to the percentages of peripheral blood eosinophils and neutrophils （ all P 〉 0. 05 ）. Serum PDCD5 was significantly higher in the patient group （ （47.7 ± 29. 6 ） vs （ 19. 3 ± 9.8 ） μg/L, P 〈 0. 05 ）. Patients of chronic and aente phases showed a significant higher expression in PDCD5 than the remission phase （（55.2±24.5） ＆ （68.5 ±22.1） vs （16.0 ±7.9） μg,/L, both P〈0.05）. Serum PDCD5 of asthmatics showed a negative correlation with FEV1%, FEV1% FVC ratio and ACT scores （r = - 0. 539 to - 0. 798, all P 〈0. 05 ）. Conclusions PDCD5 participates in the inflammatory process of asthmatic airway. Its abnormal expression may be associated with the uncontrolled state of asthmatics. It may serve as an indicator of assessing the levels of asthma control or a target for the treatment of asthma.

关 键 词：哮喘 炎症 程序化细胞死亡因子5 呼吸功能试验 问卷调查 

分 类 号：R562.25[医药卫生—呼吸系统]