子宫内膜异位症合并不孕患者种植窗期硒结合蛋白1的表达变化  

Altered expression of selenium-binding protein 1 during endometrial window of implantation in patients with endometriosis-associated infertility

作　　者：蒋亚玲[1] 李冰[1] 邢福祺[2] 王芳[1] 冯健怀[1] 

机构地区：[1]广州医学院第三附属医院妇产科,广州510150 [2]南方医科大学南方医院生殖医学中心,广州510515

出　　处：《现代妇产科进展》2012年第5期342-344,349,共4页Progress in Obstetrics and Gynecology

基　　金：广东省医学科研基金(No:A2010271);广州医学院第三附属医院青年科研支持计划(No:2011Y04)

摘　　要：目的:探讨子宫内膜异位症合并不孕患者种植窗期硒结合蛋白1(SBP1)的差异表达与子宫内膜容受性的关系。方法:采用荧光差异凝胶电泳(2D-DIGE)和基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF-MS)技术,对子宫内膜异位症合并不孕患者和输卵管因素不孕(除外子宫内膜异位症)患者种植窗时期[排卵日(OD)后6天(OD+6天)]在位子宫内膜的蛋白质表达谱进行比较研究。应用免疫印迹(Western blot)技术,对两者子宫内膜组织进行SBP1的半定量表达分析。结果:电泳图谱显示,等电点约5.93,相对分子质量为52.9kD的蛋白斑点在子宫内膜异位症合并不孕患者OD+6天的表达较对照组增加1.58倍;经鉴定,此蛋白斑点肽指纹图谱(PMF)为SBP1;其在子宫内膜异位症合并不孕患者种植窗时期的表达量较对照组显著增加,两组蛋白质相对灰度值分别为13.89±1.63和11.34±0.95(t=-2.709,P=0.035)。结论:子宫内膜异位症合并不孕患者种植窗时期SBP1表达显著上调,可能与子宫内膜对胚胎的接受性下降有关。Objective：To explore the association of altered expression of selenium-binding protein 1（SBP1）during endometrial window of implantation（WOI） in patients with endometriosis-associated infertility and endometrial receptivity.Methods：Proteomic technology,a combination of the two-dimensional fluorescence differential in-gel electrophoresis（2D-DIGE） and matrix-assist laser desorption ionization-time of flight-mass spectrometry（MALDI-TOF-MS）,was adopted to search for proteome alternations of WOI[ at Day-6 after ovulatory day（OD）,OD＋6 day]in patients with endometriosis-associated infertility versus those with tubal infertility.The semi-quantitative validation of SBP1 in the eutopic endometrial tissue during WOI was analyzed by Western blot.Results：By comparing protein profiles,we found a protein,with a isoelectric point of 5.93 and relative molecular weight of 52.9 kD,up-regulated 1.58 times in samples of WOI in patients with endometriosis-associated infertility.Using mass spectrometry,it was identified as SBP1.Protein level of SBP1 was up-regulated during WOI in patients with endometriosis-associated infertility comparing with that in control group by Western blot.Relative intensity of SBP1 were 13.89±1.63 and 11.34±0.95（t=-2.709,P=0.035）.Conclusions：It seems that the increased expression of SBP1 during WOI in patients with endometriosis-associated infertility plays an important role in the decrease of endometrial receptivity.

关 键 词：子宫内膜异位症 不孕症 蛋白质组学 硒结合蛋白1 

分 类 号：R711.71[医药卫生—妇产科学]