竞争包合荧光光度法测定诺氟沙星  

Determination of norfloxacin by competitive inclusion spectrofluorimetry

作　　者：陈世界[1,2] 李英杰[2] 苏立强[2] 王鹏[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150090 [2]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《分析试验室》2012年第6期13-15,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(50821002);齐齐哈尔大学青年教师科研启动支持计划项目(2010K-M19)资助

摘　　要：以中性红(NR)为荧光探针,利用羟丙基-β-环糊精(HP-β-CD)能够与中性红(NR)形成包合物,从而使中性红的荧光强度大大增强,而诺氟沙星的加入使NR-HP-β-CD体系的荧光猝灭,以此建立了荧光猝灭法测定诺氟沙星的新方法。在优化的实验条件下,诺氟沙星工作曲线的线性范围为:6.3×10-8～1.3×10-6mol/L,检出限为:1.9×10-8mol/L。方法可用于环境水样中痕量诺氟沙星的测定,回收率在98.4%～104.0%之间。This paper explored a novel fluorescence quenching method for the determination of norfloxacin.It was based on that the interaction between norfloxacin and hydroxypropyl-β-cyclodextrin（HP-β-CD） via a competitive includsion reaction with neutral red（NR）as a fluorescence probe.The results showed that： the addition of norfloxacin could quench the fluorescence intensity of the system（NR-HP-β-CD） under the optimal conditions,and there was had a linear relationship between the concertration of norfloxacin and the fluorescence quenching value of the system（ΔF）.The linear range of calibration curve for the determination of norfloxacin was 6.3×10-8~1.3×10-6 mol/L,and the detection limit was 1.9×10-8mol/L.

关 键 词：羟丙基-Β-环糊精 竞争包合荧光分光光度法 中性红 诺氟沙星 

分 类 号：O657.3[理学—分析化学]