液相色谱-串联质谱法测定水产品中麻醉剂MS-222残留  

Determination of tricaine residues in aquactic products by liquid chromatography-tandem mass spectrometry

作　　者：朱敏[1] 孙伟红[2] 邢丽红[2] 冷凯良[2] 苗均魁[2] 许洋[2] 贾福强[2] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003 [2]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《分析试验室》2012年第6期59-62,共4页Chinese Journal of Analysis Laboratory

基　　金：农业标准制修订项目(2010-383)资助

摘　　要：建立了液相色谱-串联质谱法测定水产品中麻醉剂3-氨基苯甲酸乙酯甲基磺酸盐(MS-222)残留量的方法。提取液为50%的甲醇及乙酸-乙酸钠缓冲溶液,提取液经C18固相萃取柱净化处理后用液相色谱-串联质谱仪进行测定,外标法定量。流动相为0.5%的甲酸溶液和乙腈(V:V=60:40),流速为0.2 mL/min。该方法的线性范围为0.001～1.0 mg/L,相关系数大于0.999,检出限为1μg/kg,定量限为2μg/kg。加标回收率可以达到80%～110%。A liquid chromatography tandem mass spectrometric(LC-MS/MS)method for the determination of residues of the anaesthetic tricaine mesilate(MS-222) in aquatic products was established.Residues were extracted from homogenized tissues with methanol at a volume ratio of 50:50 and acetic acid-sodium acetate buffer(pH 4.2).The extract passed through a C18 solid-phase extraction column followed by LC-MS/MS analysis,and quantified with an external standard method.The mobile phase was 0.5% formic acid and acetonitrile(V:V=60:40),and flow rate was 0.2mL/min.The linear range was from 0.001 to 1.0 mg/L with correlation coefficients better than 0.999.The limit of detection was 1 μg/kg and the limit of quantification was 2 μg/kg.The recoveries were in the range of 80%~110%.

关 键 词：3-氨基苯甲酸乙酯甲基磺酸盐(MS-222) 水产品 液相色谱-串联质谱 残留 

分 类 号：O657.63[理学—分析化学]