HCMV感染患儿血浆可溶性HLA-G及IL-10检测的意义  

Plasma HLA-G and IL-10 Detection in Children with Human Cytomegalovirus Infection

作　　者：田可港[1] 浮苗[1] 郑晓群[1] 

机构地区：[1]温州医学院附属第二医院检验科,325027

出　　处：《医学研究杂志》2012年第5期86-88,共3页Journal of Medical Research

基　　金：温州市对外合作交流科技计划项目(H20090077)

摘　　要：目的探讨血浆可溶性人类白细胞抗原-G(soluble human leukocyte antigen-G,sHLA-G)及白细胞介素-10(in-terleukin-10,IL-10)在儿童巨细胞病毒(human cytomegalovirus,HCMV)感染中的意义。方法分别收集75例HCMV活动性感染患儿的外周血及尿液标本,采用酶联免疫吸附试验(ELISA)测定血浆sHLA-G和IL-10水平;采用实时荧光定量PCR(FQ-PCR)检测尿液HCMV DNA载量。结果 HCMV活动性感染患儿血浆sHLA-G水平[54.91(6.75～282.72)U/ml]明显高于对照组[21.32(1.07～260.35)U/ml],差异有统计学意义(P<0.001);血浆IL-10的水平[9.24(1.61～41.77)pg/ml]明显高于对照组[1.82(1.03～3.98)pg/ml],差异有统计学意义(P<0.001);血浆sHLA-G和IL-10水平与尿液HCMV DNA载量之间无明显相关性(P>0.05)。结论 HCMV活动性感染患儿血浆sHLA-G和IL-10水平显著升高,可作为HCMV活动性感染诊断的指标。Objective To discuss the significance of plasma levels of soluble human leukocyte antigen - G （ sHLA - G） and inter- leukin - 10 （IL - 10） in children with human cytomegalovirus （HCMV） infection. Methods The study included 75 patients with active HCMV infection. Plasma levels of sHLA - G and IL - 10 were determined using enzyme - linked immunosorbent assay（ELISA） , and real - time fluorescence quantitative PCR（ FQ - PCR） was used to detect the urine HCMV DNA load. Results Significant increases were ob- served in soluble HLA - G level [54.91 （6.75 - 282.72） U/ml;P 〈 0. 001 ] and IL - 10 level [9.24（ 1.61 - 41.77） pg/ml;P 〈 0. 0011 among patients with active HCMV infection as compared with that among healthy control subjects. Furthermore, no significant correlation was established between urine HCMV DNA load and levels of sHLA - G and IL - 10 （P 〉 0.05 ）. Conclusion Plasma sHLA - G and IL - 10 levels were increased during active HCMV infection. No correlation was established between urine HCMV DNA load and levels of sHLA - G and IL - 10.

关 键 词：人巨细胞病毒人类白细胞抗原-G 白细胞介素-10 

分 类 号：R512.990.4[医药卫生—内科学]