空间诱变对紫花苜蓿过氧化物同工酶影响  

Effect of Space Flight Factors on the Peroxidase Polymorphism and Its Activity of Alfalfa

作　　者：李晶[1,2] 任卫波[1] 郭慧琴[2] 刘雅学[1] 陈立波[1] 张文静[1] 赵海霞[1] 

机构地区：[1]中国农业科学院草原研究所,内蒙古呼和浩特010010 [2]内蒙古农业大学生物工程学院,呼和浩特010018

出　　处：《种子》2012年第4期46-48,共3页Seed

基　　金：内蒙古自然基金(编号:2010 BS 0408);中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)资助项目(2011)

摘　　要：为了探索空间诱变环境对紫花苜蓿生理生化活动的影响,本研究分别利用分光光度计和聚丙烯酰胺垂直板电泳法对卫星搭载紫花苜蓿过氧化物同工酶酶带及酶浓度进行研究。结果表明,经过卫星搭载后,紫花苜蓿过氧化物酶活性提高18%,这可能是由于种子在飞行过程中受到重粒子辐射、微重力、极端温度等特殊环境刺激,使得种子内部活性氧大量累积,从而导致过氧化物酶活性上调。同时电泳结果还发现,搭载后过氧化物酶酶带发生显著变化,表现为新酶带的增加,这表明空间飞行因子不仅可以刺激酶活性的上调,还可以改变酶组成。这一发现初步揭示了卫星搭载对苜蓿酶活动的影响,有助于增加对苜蓿空间诱变效应的认识。To explore the effect of space flight factors on the physiology activity of alfalfa seedlings,the activity and band pattern of peroxidase were analyzed in this study.The results showed that the activity of peroxidase of flight seedlings increased 18% than that of ground control,which might be activated by the accumulations of active oxygen induced by space flight factors.Moreover,the band pattern of peroxidase was also changed after the space flight and new bands were observed in the band pattern of peroxidase of flight seedlings.

关 键 词：紫花苜蓿 卫星搭载 过氧化物同工酶 

分 类 号：S335.2[农业科学—作物遗传育种] S551.7[农业科学—农艺学]