原子吸收分光光度法测定骨肽注射液中钙的含量  

Content Determination of Calcium in Ossotide Injection by AAS

作　　者：宋阳[1] 宋广群[2] 茅建辉 

机构地区：[1]武警上海总队医院药剂科,上海201103 [2]武警黑龙江总队医院药剂科,哈尔滨150010

出　　处：《中国药房》2012年第23期2170-2172,共3页China Pharmacy

摘　　要：目的:建立测定骨肽注射液中钙含量的方法,并对其进行方法学验证。方法:样品采用混合酸消化后导入原子吸收分光光度计,经火焰原子化,于422.7nm波长处测定。结果:钙的检测浓度在0～8μg.mL-1范围内与吸光度呈良好的线性关系(r=0.9997);平均加样回收率为100.34%,RSD=1.97%(n=9)。结论:本方法灵敏、准确、可靠,可用于检测骨肽注射液中钙的含量。OBJECTIVE： To establish the method for the content determination of calcium in Ossotide injection, and to verify the methodology of it. METHODS： Firstly the samples were digested with mixed acid and introduced into atomic absorption spectrophotometer, and then measured by AAS with absorbance wavelength at 422.7 nm. RESULTS： The linear range of calcium was 0-8 μg-mL-1（r=0.999 7） with an average recovery of 100.34% （RSD=1.97% ,n=9）. CONCLUSION： The method is sensitive, accurate and reliable, and can be used for the content determination of calcium in Ossotide injection.

关 键 词：骨肽注射液 原子吸收分光光度法 钙 含量 

分 类 号：R283.611[医药卫生—中药学] R927.2[医药卫生—中医学]