53例人用狂犬病无佐剂纯化疫苗接种后抗体检测结果分析  

Analysis on detection results of antibody of 53 cases with a new inactivated(Vero cell)rabies vaccine

作　　者：王余俊[1] 王晓昱[1] 石建方[1] 宋婷婷[1] 张秋娥[1] 雷世光[1] 王洪飚[1] 王梅[1] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004

出　　处：《现代预防医学》2012年第11期2826-2828,共3页Modern Preventive Medicine

基　　金：贵州省卫生厅科学技术基金资助(gzwkj2008-1-044)

摘　　要：目的观察暴露后人群接种国产人用狂犬病无佐剂纯化疫苗(Vero cell)的免疫效果。方法对53例研究对象按照0、3、7、14和28 d程序进行暴露后免疫,采用WHO认可的RFFIT法检测免疫14 d,45 d的血清抗体。观察每针次接种后72 h内局部和全身反应。结果所有接种对象均未出现严重副反应。首剂免疫14 d,45 d ELISA法检测抗体阳性率分别为52.83%,94.34%,RFFIT法检测抗体阳性率分别为100%,100%。几何平均滴度(GMT)为17.74 IU/ml,15.50 IU/ml。结论国产人用狂犬病无佐剂纯化疫苗(Vero cell)具有良好的安全性和免疫原性。OBJECTIVE To explore immune effect by post-exposure a new inactivated（Vero cell）rabies vaccine.METHODS 53 healthy volunteers were inoculated with rabies vaccine prepared from Vero cells immunization,which was performed at day 0,3,7,14 and 28.Seroconversion and geometric mean titer（GMT）of antibody to the virus of rabies were determined at day 14 and 45 after first dose of vaccination by ELISA and RTTIT methods.RESULTS Local and systemic serious reaction were not observed in all participates.After the first dose of immunization,the positive rate of IgG,measured by the ELISA method at day-14 and 45 were 52.83% and 94.34% respectively.Using the RFFIT method,the positive rate of antibody at day-14 and 45 was 100% and 100% respectively.The titers of antibody were 17.74IU/ml and 15.50IU/ml.CONCLUSION The home-made inactivated rabies vaccine（Vero cell）is safe and highly immunogenic.

关 键 词：疫苗 狂犬病毒抗体 酶联免疫吸附试验 快速免疫荧光灶抑制试验 

分 类 号：R186[医药卫生—流行病学]