东祁连山高寒草地纤维素分解菌的分离和筛选  

Isolation and screening of cellulose-decomposition fungi in alpine grassland on eastern Qilian Mountains

作　　者：刘雯[1] 卞静[1] 付美涛[1] 陈秀蓉[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2012年第2期78-81,86,共5页Grassland and Turf

基　　金：大学生科研训练项目(201002005);甘肃省自然科学基金(O96RJZA003)资助

摘　　要：从东祁连山高寒草地土壤中分离真菌菌株,以CMC-Na培养基,刚果红染色法和滤纸平板培养基为指标对分离的菌株进行纤维素分解菌的初步筛选,然后,通过测定滤纸减重率和CMC酶活力进行复选,获得了3株纤维素分解能力较强的菌株F-3,F-25和F-22,表明高寒草地土壤中具有较强分解能力的纤维素分解菌。The soil samples of alpine meadow on the eastern Qilian Mountains were collected to isolate the cellulose-decomposing fungi strains with CMC-Na plate culture medium in combination with a filter paper plate culture medium.The loss of filter paper and the CMC enzyme activity were used as the indices to conduct further screening.3 cellulose decomposition fungi strains were obtained and coded as F-3,F-25 and F-22.

关 键 词：东祁连山 纤维素分解菌 分离 

分 类 号：S812[农业科学—草业科学]