香蕉枯萎病的离体叶片接种技术研究  

Research on in vitro leaves inoculation technique for banana Fusarium Wilt Disease

作　　者：彭铁成[1] 陈云云[1] 罗静瑶[1] 谢俊[1,2] 汤华[1,2,3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]海南热带生物资源可持续利用国家重点实验室培育基地,海南海口570228 [3]热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《广东农业科学》2012年第10期7-9,F0002,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30860149);教育部科学技术研究重点项目(210172)

摘　　要：香蕉枯萎病是一种毁灭性真菌病害,对香蕉的种植和产业发展危害严重,其病原菌为尖孢镰刀菌古巴专化型(Fusariumoxysporum f.sp.cubense)。为研究该病原菌的致病机理和香蕉的枯萎病抗性机理,对香蕉枯萎病的离体叶片接种方法进行了研究,结果表明:香蕉叶片在离体条件下必须要有伤口才能被侵染发病,枯萎病菌4号小种的菌丝和分生孢子溶于无菌水或者PDB溶液都能引起发病;在一定浓度范围内,发病程度与刺孔数量和细菌浓度成正比;在刺孔条件下,香蕉枯萎病分生孢子浓度为0.9×105个/mL时的接种效果比较显著。Fusarium wilt of banana is a destructive fungus disease caused by Fusarium oxysporum f. sp. Cubense. This disease had harmful effects on banana planting and its industry development. In order to study the disease-causing mechanism of pathogen and the disease-resistant mechanism of banana, it was necessary to isolate and identify the pathogenic fungus. In this research, the in vitro inoculation methods of Fusarium wilt on banana leaves were investigated. The results showed that the in vitro banana leaves can be infected from injured point; the disease degree was related to punctured holes and fungi concentration; the pathogenic effect of the punctured banana leaves was signficant when the conidium concentration of Fusarium oxysporum f. sp. Cubense was 0.9×10^5 cells/mL.

关 键 词：香蕉 枯萎病 尖孢镰刀菌古巴专化型 离体接种技术 

分 类 号：S432.4[农业科学—植物病理学]