基于18S rDNA序列的涡虫纲三肠目分子系统发育研究  

Molecular phylogeny of Turbellarian Tricladida inferred from 18S rDNA

作　　者：付瑞敏[1,2,3] 蔺芳[4] 常慧萍[2,3] 张红[2,3] 张丽琴[2,3] 邢文会[2,3] 韩鸿鹏[2,3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南教育学院人口与生命科学系,河南郑州450046 [3]微生物资源开发与应用研究所,河南郑州450046 [4]新乡学院生命科学与技术系,河南新乡453003

出　　处：《广东农业科学》2012年第10期148-150,154,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31170357);河南省杰出青年基金(0312001100);河南教育学院青年科研课题(20100103);河南省教育厅自然科学研究计划项目(2011B180015);河南省科技厅科技攻关项目(122102310171)

摘　　要：采用18S rRNA对我国常见的4种三肠目涡虫的18S rDNA基因进行序列测定和分析,共获得三肠目2科3属4种的序列,同时结合GenBank中的三肠目18S rDNA序列(22条),以其姐妹群原序列目中的Archimonocelis crucifer为外群,采用MEGA3.0、PHYLIP、PAUP 4.0等分子生物学软件分别构建NJ树、ME树、MP树和ML树,分析三肠目的系统发育关系。结果表明,不同构树方法所得系统树的拓扑结构基本一致。In order to investigate system development relationship Turbellarian tricladida in the molecular level, fragments of 18S rDNA, as gene marker, were sequenced for 4 species from 3 genera of the Paludicola, and the homologous sequences of Tricladida downloaded fi＇om GenBank. After alignment for these sequences of 22 species of Tricladida with choosing Archimonocelis crucifer as outgroup, the phylogenetic relationships among 22 species of three suborders of Tricladida were studied. Meanwhile, four phylogenetic trees, such as NJ tree, ME tree, ML tree and MP tree, were reconstructed with some molecular biology software that was MEGA 3.0, PHYLIP and PAUP 4.0. Subsequently, the topologv of four phvlogenetic trees were found to be consensus on the whole.

关 键 词：涡虫纲 三肠目 分子系统发育 18S rDNA 

分 类 号：Q959.151[生物学—动物学]