2458名中国汉族人类HPA-1～6、15基因多态性的研究  

The polymorphism of HPA-1～6、15 in Chinese Han population

作　　者：周丹[1] 张印则[1] 庄乃保[1] 孔令魁[1] 

机构地区：[1]深圳巿血液中心,广东深圳518035

出　　处：《中国输血杂志》2012年第4期300-303,共4页Chinese Journal of Blood Transfusion

基　　金：深圳市科技局科研基金项目(201003200)

摘　　要：目的分析中国汉族人群HPA-1～6、15系统的基因多态性,研究中国南、北方汉族人群HPA-1～6、15的基因分布。方法采用Luminex结合序列特异性寡核苷酸探针(flow-sequence specific oligonucleotide probes,FLOW-SSO)方法对2 458名深圳巿机采血小板无偿捐献者(其中南方人群1 554人,北方人群904人)进行HPA-1～6,15系统基因分型。采用聚合酶链反应-序列特异性引物(PCR-sequence specific primer,PCR-SSP)方法对有疑问的标本及罕见抗原(如HPA-1b/1b、2b/2b、5b/5b等)做进一步确认。结果中国汉族人群中HPA-1～6、15基因频率分别为,HPA-1a 0.991 7,HPA-1b 0.008 3,HPA-2a 0.955 2,HPA-2b 0.044 8,HPA-3a 0.558 6,HPA-3b 0.441 4,HPA-4a0.998 0,HPA-4b 0.002 0,HPA-5a 0.986 2,HPA-5b 0.013 8,HPA-6a 0.985 6,HPA-6b 0.014 4,HPA-15a 0.545 2,HPA-15b 0.454 8。南、北方汉族人群HPA-1～6、15比较,在HPA-1和HPA-3系统(χ2=15.032 0、5.418 8,P<0.05);基因频率分布差异有统计学意义。经χ2检验,符合Hardy-Weinbery遗传定律。结论中国汉族人群中HPA-3和HPA-15杂合程度最高,在中国南北方汉族人群中HPA-1和HPA-3系统基因多态性分布存在明显的地域差异。为了给免疫性血小板减少症患者提供相合血小板输注,在中国建立血小板供者资料库是必要的。Objective To analyze human platelet antigens （HPA） 1-6,15 polymorphism, and study the allele frequencies in the North Chinese Han and South Chinese Han. Methods The genotypes of HPA1-6,15 systems were performed by FLOW-SSO for 2 458 samples. Results The gene frequencies were 0. 991 7,0. 008 3,0. 955 2,0. 044 8,0. 558 6,0. 441 4,0. 998 0,0. 002 0,0. 986 2,0. 013 8,0. 985 6,0. 014 4,0. 545 2,0. 454 8 for HPA-1 a, HPA-1 b, HPA-2a, HPA-2b, HPA- 3 a, HPA-3b, HPA-4a, HPA-4b, HPA-5 a, HPA-5b, HPA-6a, HPA-6b, HPA-15a, HPA-15b, respectively. The phenotype frequencies of HPA1-6,15 in the South Chinese Han was compared to the frequencies in the north Chinese Han. There is significant deviation from Hardy-Weinbery equilibrium within HPA-1,3 in the south Chinese Han and north Chinese Han（χ^2 = 15. 032 0,5. 418 8,P 〈 0.05）. There was a good fit to Hardy-Weinbery equilibrium within each group. Conclusion The highest heterozygotes are HPA-3 and HPA-15 ,which indicate that the original difference of HPA-1 and HPA-3 in South and North of China. In order to provide HPA-matched platelet transfusion in alloimmune thrombocytopenic patients,the building of China HPA-typed plateletpheresis donor registry is very useful.

关 键 词：中国汉族人群 人类血小板抗原 FLOW—SSO 基因频率 

分 类 号：R457.11[医药卫生—治疗学] R331.143[医药卫生—临床医学]