钆-二乙三胺五乙酸与牛血清白蛋白作用的核磁共振研究  

Studies of Gd-Diethylenetriamine Pentaacetic Acid in Boving Serum Albumin Solution by Nuclear Magnetic Resonance Spectroscopy

作　　者：李晓晶[1] 冯江华[1] 李欣宇[1] 吴亦洁[1] 裴奉奎[1] 

机构地区：[1]中国科学院长春应用化学研究所应用谱学开放实验室,长春130022

出　　处：《分析化学》2000年第3期269-272,共4页Chinese Journal of Analytical Chemistry

基　　金：中国科学院"九五"预研课题

摘　　要：采用核磁共振弛豫分析法研究了造影剂母体化合物钆-二乙三胺五乙酸（Gd-DTPA）在水溶液和牛血清白蛋白溶液中的诱导弛像增强性质。Gd-DTPA络合物在水溶液中弛豫效率为5．25mmol-1·L·s-1，并发现牛血清白蛋白分子可非共价地结合Gd－DTPA络合物，这种非共价结合体的旋转相关时间明显长于自由络合物，使Gd-DTPA在蛋白质溶液中弛豫效率增高。本文结果表明该方法可以用来研究顺磁性金属络合物与蛋白质之间的相互作用情况。In this paper, the water relaxation enhancemen behavior of Gd-diethylenetriamine pentaacetic acid(DTPA) in water and in aqueous solution of bovine serum albumine (BSA) has been studied. The T1relaxivity of Gd-DTPA in BSA solution is higher than that in aqueous solution. The results indicate that Gd-DTPA can integrate non-covalently with BSA mainly in forms of (Gd-DTPA)·BSA、 (Gd-DTPA)2·BSA, for which the apparent equilibrium constant is 0.026 mmol-1·L、0.0018 mmol-2·L2 respectively.This method would be used to study the interactivities between protein and contrast agent.

关 键 词：钆 牛血清白蛋白 弛豫性能 核磁共振 GD-DTPA 

分 类 号：O657.2[理学—分析化学]