高效毛细管区带电泳法分离人血清蛋白质的研究  

Study on the Separation of Proteins in Human Serum by High Performance Capillary Zone Electrophoresis

作　　者：李克[1] 袁倚盛[1] 赵飞浪[1] 

机构地区：[1]南京军区南京总医院全军医学检验中心,江苏南京210002

出　　处：《色谱》2000年第2期152-154,共3页Chinese Journal of Chromatography

摘　　要：研究了高效毛细管区带电泳分离人血清蛋白质的电泳行为及实验条件 ,建立了分离血清蛋白质的高效毛细管区带电泳法。血清样品经硼酸缓冲液 (5 0 mmol/L,p H8.80 )稀释后 ,以 0 .1 mol/L硼酸缓冲液 (p H9.3 5 ,含4g/L PEG80 0 0 )为电泳介质 ,在 5 0 μm i.d.× 3 7cm弹性石英毛细管柱 (有效柱长为 3 2 cm)中进行电泳分离 ,以2 0 0 nm紫外波长检测。方法简便、快速、重复性好 ,1 2 min内便可完成对血清中蛋白质的电泳分离。A high performance capillary zone electrophoretic method has been developed for the separation of proteins in human serum. Sera were diluted with a buffer (0.05 mol/L boric acid, pH 8.80), and then introduced hydrodynamically into a 37 cm(32 cm to detector)×50 μm i.d. fused\|silica capillary, electrophoresed for 12 min using a boric buffer solution (0.10 mol/L, pH 9.35; containing 4 g/L of polyethylene glycol 8000), and detected at the wavelength of 200 nm. The method is simple, rapid and repeatable.

关 键 词：毛细管区带电泳 蛋白质 血清蛋白 分离 

分 类 号：Q510.3[生物学—生物化学] Q592.1