茯苓三萜成分RP-HPLC-ELSD指纹图谱研究  

RP-HPLC-ELSD fingerprint of triterpenes in Poria

作　　者：昝俊峰[1] 徐斌[1] 刘军锋[1] 苏玮[2] 王克勤[2] 刘焱文[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,湖北武汉430061 [2]湖北省中医药研究院,湖北武汉430073

出　　处：《中草药》2012年第5期895-900,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30970295);湖北省教育厅青年基金项目(鄂教Q20091606)

摘　　要：目的利用RP-HPLC-ELSD技术建立不同菌株茯苓三萜的指纹图谱,为评价不同菌株培育茯苓药材的质量品质提供理论依据,以期规范培育茯苓的菌株,提高茯苓资源品质。方法色谱柱为Kromasil 100-5 C18柱(250 mm×4.6 mm,5μm);流动相为0.2%甲酸水溶液(A)-乙腈(B),采用梯度洗脱法;体积流量为1.0 mL/min;柱温为30℃;蒸发光检测条件:雾化温度为40℃,压力为350 kPa,gain值为7;进样量为20μL。结果建立的指纹图谱中有18个共有峰,且方法学考察符合规定的标准。结论该方法简便快速、准确可靠,可作为评价不同菌株茯苓药材质量的有效手段。Objective Investigation on RP-HPLC-ELSD fingerprint of triterpenes in Poria from 27 strains,in order to provide a theoretical evidence to evaluate the quality of Poria from different strains,get the normative bacterial strains,and improve the quality of Poria.Methods The chromatographic conditions were Kromasil 100-5 C18column（250 mm × 4.6 mm,5 μm）,0.2% methanoic acid（A） and acetonitrile（B） as gradient mobile phases,flow rate being 1.0 mL/min,and column temperature at 30 ℃.Evaporative light-detection conditions： atomization temperature at 40 ℃,pressure of 350 kPa,gain value of 7,and 20 μL sample injection.Results Fingerprint was established with 18 common peaks and methodology reached the required standard.Conclusion The method is simple,rapid,accurate,and can be used as an effective means of Poria quality evaluation from different strains.

关 键 词：菌株 茯苓 三萜 RP-HPLC-ELSD色谱法 指纹图谱 

分 类 号：R286.02[医药卫生—中药学]