不同产地仙茅遗传多样性的ISSR分析  

Analysis on genetic diversity of Curculigo orchioides from different habitats by ISSR

作　　者：李隆云[1] 陈大霞[1] 钟国跃[1] 李泉森[1] 

机构地区：[1]重庆市中药研究院,中药种植研究所,重庆市中药良种选育与评价工程技术研究中心,重庆400065

出　　处：《中草药》2012年第5期967-971,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技攻关计划项目(2001BA701A32);国家科技攻关计划项目(2004BA604A04);重庆市科技计划项目(6455)

摘　　要：目的从DNA水平上对仙茅种质资源进行遗传多样性分析。方法对我国25个主要分布区的仙茅进行ISSR-PCR扩增与检测。扩增结果转化为"0"、"1"矩阵后,利用Treeconw软件计算遗传距离,并采用UPGMA聚类法构建亲缘关系树状图。结果 14条引物共得到176条清晰可辨的扩增条带,其中有151条呈现多态性,占85.8%。遗传距离变化范围在0.177 2～0.403 0。25份样品分为两大类,聚类较为复杂,大多种质不具明显地理相关性,仅在小分支中呈现出一定的地域性分布规律。结论我国仙茅植物的遗传多样性十分丰富,为筛选优质种质资源提供了丰富的遗传基础。Objective To analyze the genetic diversity of Curculigo orchioides from different habitats at DNA molecular level.Methods C.orchioides from 25 germplasmic resources was analyzed by ISSR-PCR.After translating the amplification results into ＂0＂ and ＂1＂ matrix,the genetic distances were calculated by Treeconw software and the systematic diagram of genetic relationship was clustered by UPGMA method.Results A total of 176 bands were detected using 14 primers,among which 151 were polymorphic bands.The average percentage of polymorphic bands was 85.8%.Genetic distances were changed from 0.177 2 to 0.403 0.Clustering results showed that the genetic relationship of C.orchioides was more complex.The correlation between the most germplasm and its geographic origin was not very obvious,except a few of C.orchioides from the same region being in the same group,and followed the law of geographical distribution in the tested materials.Conclusion Significant polymorphism and genetic diversity are observed among C.orchioides germplasm resources which could provide a wealth of genetic basis for the germplasm high quality.

关 键 词：仙茅 ISSR 种质资源 遗传多样性 多态性 

分 类 号：R282.12[医药卫生—中药学]