烟夜蛾滞育蛹和非滞育蛹血淋巴可溶性蛋白含量及图谱初步研究  

A Preliminary Study on the Content and Electrophoresis Patterns of Soluble Protein in Hemolymph from Diapause and Non-diapause Pupae of Helicoverpa Assulta

作　　者：高玉红[1,2] 王冬燕[1,3] 郭线茹[1] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]郑州职业技术学院,河南郑州450121 [3]河南省汝阳县内埠镇政府,河南汝阳471200

出　　处：《江西农业大学学报》2012年第2期294-297,323,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：河南省教育厅自然科学基金项目(2006180010)

摘　　要：利用考马斯亮蓝法和SDS-PAGE电泳法测定并分析了烟夜蛾滞育蛹和非滞育蛹血淋巴可溶性蛋白含量及其组成。结果表明,非滞育蛹在化蛹后至羽化前可溶性蛋白呈现出"降低—升高—降低"的变化趋势,在5日龄时含量最高,而滞育蛹呈现出持续降低的变化趋势;除2日龄雌蛹外,其他日龄滞育与非滞育蛹的雌、雄个体间蛋白质含量均存在显著差异。蛋白质SDS-PAGE电泳图谱表明,滞育蛹与非滞育蛹血淋巴的蛋白组成存在明显差异。推测滞育蛹血淋巴可溶性蛋白含量及组成的变化与其抗寒性有关。The content and composition of soluble protein in the hemolymph from diapause and non-diapause pupae of Helicoverpa assulta were measured and analyzed by using Bradford and SDS-PAGE.The results showed that,the change tendency of soluble protein content in the non-diapause was down-up-domn from pupation to eclosion,and the largest content appeared on the fifth day after pupation,but the content in the diapause pupae decreased continuously.Except for 2-day female pupae,there were significant differences in the soluble protein content between the female and male pupae.The electrophoretograms of SDS-PAGE indicated that there were signficant differences in the composition and content of soluble protein between diapause and non-diapause pupae.So it could be deduced that these changes of soluble protein of hemolymph in the diapause pupae were related to the cold-resistance of H.assulta.

关 键 词：烟夜蛾 滞育 血淋巴 可溶性蛋白 

分 类 号：Q969.436.4[生物学—昆虫学]