GM-CSF与生长抑素融合表达质粒对小鼠肌肉组织GHR和IGF-I mRNA表达的影响  

GHR and IGF-I mRNA Expression in Muscle Tissue of Mice after Immunization with Fusion-Expression Plasmid Contained GM-CSF and SS

作　　者：舒邓群[1] 朱晓华[2] 吴红翔[1] 王开云[1] 曾检华[1] 魏金刚[1] 胡豆 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045 [2]江西科技师范学院,江西南昌330013

出　　处：《江西农业大学学报》2012年第2期339-344,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省教育厅科技项目(GJJ08187);江西农业大学博士启动基金

摘　　要：选择70只小白鼠,随机分为7组,每组10只,雌雄各半。其中第1组为对照组,第2-4组分别肌肉注射pcS/2SS、pGM-CSF/SS和pGM-CSF+pcS/2SS DNA疫苗;第5～7组分别口服以减毒沙门氏菌为载体的pcS/2SS、pGM-CSF/SS和pGM-CSF+pcS/2SS DNA疫苗,以β-actin作为内参,利用相对半定量RT-PCR,检测小鼠肌肉组织GHR和IGF-I mRNA的表达。结果表明:第3、5和6组的GHR mRNA表达显著高于1组和7组(P<0.05),第5和6组的IGF-I mRNA表达显著高于1组、2组、4组和7组(P<0.05),GM-CSF与SS的融合表达质粒(pGM-CSF/SS)的GHR和IGF-I mRNA表达高于pGM-CSF+pcS/2SS共同免疫,同种DNA疫苗,口服免疫组的GHR和IGF-I mRNA表达总体上比肌肉注射组要高。这些结果证明,GM-CSF可促进SS DNA疫苗的免疫效果,提高肌肉组织中GHR和IGF-I mRNA的表达;pGM-CSF/SS效果优于pGM-CSF+pcS/2SS,口服免疫组要优于肌肉注射免疫组。A total of 70 mice at 22 days age were randomized to 7 groups to detect GHR and IGF-I mRNA expression in muscle tissues by semi-quantitative RT-PCR,and β-actin was served as the control.All mice of 7 groups were given twice immunizations at intervals of 2 weeks.Group 1 as the control was immunized with 0.85 % salt solution.Group 2-4 were given muscular injections（i m.）with pcS/2SS（Group 2）,pGM-CSF/SS（Group 3） and pGM-CSF＋pcS/2SS（Group 4）,respectively,while Group 5-7 by oral administration with pcS/2SS（Group 5）,pGM-CSF/SS（Group 6） and pGM-CSF＋pcS/2SS（Group 7） delivered by attenuated Salmonella.The results were as follows： GHR mRNA expression levels of Group 3,5 and 6 were higher than that of Group 1 and 7（P0.05）.IGF-I mRNA expression levels of Group 5 and 6 were higher than that of Group 1,2,4 and 7（P0.05）.GHR and IGF-I mRNA expression levels of pGM-CSF/SS were higher than that of co-immunization of pGM-CSF ＋ pcS/2SS.The results also showed that GHR and IGF-I mRNA expression levels by the oral administration were largely higher than that of the muscular injection with the same DNA vaccine.These results suggest that GM-CSF can enhance immune effect of SS DNA vaccine and increase GHR and IGF-I mRNA expression in muscle tissue of mice.The immune effect of pGM-CSF/SS is superior to that of pGM-CSF＋pcS/2SS.The immune response of oral administration is higher than that of the muscular injection.

关 键 词：粒细胞-巨噬细胞集落刺激因子 生长抑素(SS) DNA疫苗 生长激素受体 胰岛素样生长因子-1 MRNA表达 

分 类 号：Q782[生物学—分子生物学]