半枝莲多糖的纯化及抗肿瘤作用的体内实验研究  

Experimental Study on Anti-tumor Effects of Scutellaria barbata D.Don Polyaccharides in Vivo

作　　者：杨姗姗[1] 吴大军[1] 张秀娟[2] 

机构地区：[1]淮安信息职业技术学院,江苏淮安223003 [2]哈尔滨商业大学生命科学与环境科学研究中心生物安全评价研究所,国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《江西农业大学学报》2012年第2期392-396,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：黑龙江省自然科学基金资助项目(D201043)

摘　　要：初步探究半枝莲多糖的纯化方法及其对动物体内的抑瘤作用。通过Sevag法纯化半枝莲多糖,并采用苯酚-硫酸法测定多糖含量;动物移植性肿瘤实验观察半枝莲多糖对小鼠体内肿瘤细胞生长和免疫器官—胸腺、脾的影响。多糖含量提高到49.99%;对S180肉瘤生长的抑制率以中剂量为佳;各剂量组均对脾脏指数有显著性差异,但大剂量注射(200 mg/kg),对胸腺有抑制作用。Sevag法处理后的纯多糖含量与粗多糖中的多糖含量相比有明显差异,该法切实可行;半枝莲多糖对S180小鼠有抗肿瘤作用和免疫增强作用。Objective：To study the purifying method for Scutellaria barbata D.Don polyaccharides（SPS） and its anti-tumor effect of SPS in entrails.Methods： Using Sevag and Phenol-sulfuric acid method the content of polysaccharide was determined;the anti-tumor effect of SPS and the spleen index and the thymus index were observed by tumor transplant experiment on animal.Results：SPS can inhibite the growth of S180 and the effect on middle dose group was the best;the spleen index was affected remarkably in every group and the thymus index was inhibited by big dose（200 mg/kg）.Conclusion：The content of polysaccharide purified with Sevay method and the polysaccharide content in crude polysaccharide were significantly different;the method is practical;SPS has anti-tumor effect and can improve immunity.

关 键 词：半枝莲 多糖纯化 抗肿瘤 

分 类 号：S681.9[农业科学—观赏园艺]