用反相高效液相色谱法分离和测定丁烯二酸的顺反异构体  

Determination of Two Butendioic Acid Isomers by Reversed-phase High -performance Liquid Chromatography

作　　者：李金昶[1] 赫奕[2] 孙颜 王广[1] 崔秀君[1] 

机构地区：[1]东北师范大学分析测试中心,吉林长春130024 [2]吉林工业大学应用化学系,吉林长春130025

出　　处：《分析测试学报》2000年第2期72-74,共3页Journal of Instrumental Analysis

摘　　要：建立了分离和测定丁烯二酸的2个异构体———顺丁烯二酸和反丁烯二酸的反相高效液相色谱法。以Shim - packCLC -ODS柱为分析柱 ,以甲醇 -水 (体积比5∶2 ,用磷酸调至 pH3.0)为流动相 ,流速0.7mL/min,检测波长为220nm,外标法定量。顺、反丁烯二酸的检出限分别为0.10和0.075mg/L(S/N=2) ;相对标准偏差分别为0.47 %和0.54 % (n=3)。二者的线性范围均为10～60mg/L。该法具有简便、快速、灵敏度高的特点 ,用于实际样品测定,其结果令人满意。A method for the determination of two butendioic acid isomers by reversed _ phase high _ performance liquid chromatography is described. cis _ Butendioic and trans _ butendioic acids were separated by Shim-pack CLC-ODS column, using methanol-water(5∶2 by volume)solution adjusted to pH 3.0 by phosphoric acid as a mobile phase with flow rate of 0.7 mL/min. The quantitative results are obtained by external standard method. The detection wavelength of 220 nm is applied for the samples. The detection limits are 0.1 mg/L and 0.075 mg/L(S/N=2), and relative standard deviations are 0.47% and 0.54%(n=3) for cis _ and trans _ butendioic acid, respectively. Linear range for both isomers is in 10～60 mg/L. The method is simple, rapid and sensitive, and was applied to determination of practical samples with satisfactory results.

关 键 词：高效液相色谱法 顺丁烯二酸 反丁烯二酸 异构体 

分 类 号：O623.614[理学—有机化学] O657.72[理学—化学]