HPLC同时测定小儿清解颗粒中绿原酸和连翘苷的含量  

Determination of Chlorogenic Acid and Forsythin in Xiaoer Qingjie Keli by HPLC

作　　者：黄平权 黄燕萍[2] 

机构地区：[1]广西盈康药业有限责任公司,广西南宁530023 [2]广西北海食品药品检验所,广西北海536000

出　　处：《中国民族民间医药》2012年第9期47-48,50,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立HPLC法小儿清解颗粒中绿原酸和连翘苷的含量测定方法。方法:采用Shim-pack VP-ODS(250×4.6mm)色谱柱;流动相为乙腈-0.2%磷酸溶液,梯度洗脱,流速为0.80mL.min-1,检测波长:277nm。绿原酸和连翘苷与其相邻质峰能完全分离。结果:绿原酸和连翘苷的线性范围分别为:7.30～219g.ml-1(r=0.9999)、2.35～118g.ml-1(r=1.0000)。方法回收率均不低于98%。结论:本方法简便、准确、重现性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。Objective： A HPLC method was established for the determination of Chlorogenic Acid and Forsythin in Yankening Pian. Methods： Shim - pack VP - ODS （250 × 4. 6mm） column was used with the mobile phase of acetonitrile - 0. 2% phosphoric acid solution by gradient elutio, The flow rate was 0. 80ml . ml - 1. The detection wavelength was 277nm. Chlorogenie Acid and Forsythin could be completely separated from interfacing impurity peaks. Results： The HPLC method showed agood linear relationship in the range of 7. 30 . 219g . ml - 1 （ r = 0. 9999） for Chlorogenic Acid, 2. 35 ~ 118 g . ml - 1 （ r = 1. 0000） for Forsythin. Recoveries for the four components were all above 98%. Condusion： The methods is simple accurate and with a good reproducibility and can be used for the quality control of the granules.

关 键 词：HPLC 绿原酸 连翘苷 小儿清解颗粒 

分 类 号：R927.2[医药卫生—药学]