荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定  

Spectrophotometric Determination of Piperlonguminine Based on The Charge Transfer Reaction Between Piperlonguminine and o-Nitrophenyl Fluorone

作　　者：敖登高娃[1] 安娜[1] 领小[1] 苗澍[1] 

机构地区：[1]内蒙古大学化学化工学院,内蒙古呼和浩特010021

出　　处：《发光学报》2012年第5期558-561,共4页Chinese Journal of Luminescence

基　　金：国家自然科学基金(20965004);内蒙古自然科学基金(2010BS1203)资助项目

摘　　要：建立了一种测定荜茇宁的方便快捷的荷移分光光度法。荜茇宁和邻硝基苯基荧光酮在中性三酸缓冲液中发生电荷转移反应,荷移络合物在515 nm处有最大吸收,表观摩尔吸光系数为6.571×104L.mol-1.cm-1。该络合物的组成是1∶1,药物质量浓度在0.054 6～10.92μg/mL范围内服从比尔定律,检出限为0.015 3μg/mL。对反应机理进行了初步探索,并利用本方法对生物样品中的荜茇宁进行了加标回收,回收率为99.8%～100.9%,相对标准偏差(RSD)为0.90%。A rapid and convenient spectrophotometric method for the determination of piperlonguminine was established in the present work.The method utilized charge transfer reaction between piperlonguminine and o-nitrophenyl fluorone in pH 7.00 buffer solution.The maximum absorption wavelength of the charge transfer complex was at 515 nm and the apparent molar absorptivity was 6.571×104 L·mol-1·cm-1 with a 1∶1 stoichiometry.Beer＇s law was obeyed in the range of 0.054 6~10.92 μg/mL of piperlonguminine with the detection limit of 0.015 3 μg/mL.The reaction mechanism was discussed preliminarily.The proposed method was applied to the biological and urine samples spiked with standard piperlonguminine solution.The recovery of the samples was within the range of 99.8%~100.9% and relative standard deviation（RSD） was 0.90%.

关 键 词：电荷转移反应 邻硝基苯基荧光酮 荜茇苧 分光光度法 

分 类 号：O657.32[理学—分析化学]