不同化学药剂处理对金铁锁(Psammoilene tunieoides W.C.Wu et C.Y,Wu)种子萌发的影响  

Germination of Psammoilene tunieoides Seeds with Treatments of Different Chemicals

作　　者：何丽萍[1] 李龙根[1] 钱建双[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2012年第3期340-345,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省自然科学基金项目资助(2011FZ090)

摘　　要：珍稀濒危药用植物金铁锁(Psammoilene tunieoides W.C.Wu et C.Y,Wu)种子经浓度为25%次氯酸钠(NaClO)溶液消毒处理和利用0.01%赤霉素(GA3)处理后,置于恒温20,25℃和变温20℃(16 h)～30℃(8 h)条件下进行发芽试验,探寻金铁锁种子的最适发芽温度和发芽习性;利用0.1%,0.5%,1.0%的硝酸钾(KNO3)溶液和10%,20%,30%的PEG6000溶液,分别渗透调节引发处理金铁锁种子,时间分别为8,12 h和16,24,32 h,引发温度为20℃,对经引发处理后的金铁锁种子进行发芽试验,比较上述不同化学药剂和引发处理对金铁锁种子发芽力和种子活力的影响。结果表明:金铁锁种子萌发的最适温度为恒温25℃,第15天发芽结束时发芽率为86%;经0.01%GA3,KNO3和PEG6000溶液渗透调节引发处理金铁锁种子都能显著地提高金铁锁种子的发芽力和种子活力(P<0.05),其中0.1%的KNO3渗透引发处理8,12 h和20%的PEG6000渗透引发处理24 h,金铁锁种子的发芽势、发芽率、发芽指数、活力指数等指标均达到了最大值。Seeds of Psammosilene tunicoides treated with 25% sodium hypochlorite （NaC10） for steri- lizing and 0. 01% gibberellin acid （GA3）, were germinated at constant temperatures of 20, 25% and fluctuate temperature of 20℃ （16 h） -30℃ （8 h） for the optimum germination condition. P. umicoides seeds were osmotic primed by 0. 1% , 0. 5% , 1.0% KNO3 for 8, 12 h, respectively; 10% , 20%, 30% PEG6000 for 16, 24, 32 h at 20℃, respectively. The results showed that： The optimum temperature for seed of P. tunicoides germination was 25℃ with 80% germination at the 15 d; germination rate was improved significantly （P 〈 0. 05 ） by 0.01% GA3 and 25% NaC10 treatment compared with the untreated seeds. P. tunicoides seed germination energy, germination rate, germination index, vigor index, root and shoot lengths were improved significantly by osmotic primed with KNO3 and PEG6000, especially 0. 1% KNO3 for 8, 12 h and 20% PEG6000 for 24 h osmotic priming was the best treatment with the highest germination rate, germination energy, germination index and seed vigor index compared with the other treatments.

关 键 词：金铁锁 温度 渗透调节 种子发芽力 种子活力 

分 类 号：S567.239.041[农业科学—中草药栽培]