EOS与COX-2在阿司匹林三联征患者鼻息肉组织中的表达及意义  

Expressions of EOS and COX-2 in nasal polyps in patients with aspirin triad syndrome and its significance

作　　者：李化静[1] 单志桂[2] 王玻玮[3] 刘立中[1] 

机构地区：[1]新疆医科大学第一附属医院耳鼻咽喉-头颈外科,乌鲁木齐830054 [2]新疆医科大学第一附属医院科教中心 [3]新疆医科大学第一附属医院病理科

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第11期483-487,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨嗜酸粒细胞(EOS)的聚集和COX-2在阿司匹林三联征(ST)发病过程中的调控作用。方法:取94例鼻内镜手术患者鼻息肉组织,其中ST患者34例(ST组),慢性鼻窦炎鼻息肉伴哮喘患者30例(ATA组),慢性鼻窦炎鼻息肉患者30例(对照组),应用苏木精-伊红染色及免疫组织化学SP法检测EOS的分布及COX-2的表达,并经统计学分析EOS、COX-2的表达和分布与临床病理和发病机制的关联。结果:EOS在3组患者鼻息肉组织中均大量浸润,EOS计数均数分别为80.02±6.11、76.62±5.22、65.97±4.78,ST组、ATA组分别与对照组比较差异均有统计学意义(均P<0.05),而ST组与ATA组比较差异无统计学意义(P>0.05)。COX-2在鼻息肉组织中主要表达于黏膜下腺体、腺上皮细胞、上皮细胞、血管内皮细胞及间质中的EOS,阳性细胞计数分别为88.13±6.26、89.46±5.97、91.22±4.11,ST组与对照组表达差异有统计学意义(P<0.05);ATA组表达与对照组比较差异无统计学意义(P>0.05);ST组与ATA组比较差异无统计学意义(P>0.05)。结论:EOS浸润程度不同可能是ST患者接触非甾体抗炎药后发生特有临床表现的炎症基础,COX-2在ST患者息肉组织中表达的差异可能与花生四烯酸代谢途径的转换及鼻息肉的形成有关。Objective：To explore the regulatory role of COX-2 and aggregation of EOS in the pathogenesis of Aspirin triad syndrome.Method：There were 34 patients with ST（group 1）,30 patients with chronic sinusitis and nasal polyps and asthma（group 2）,30 patients with sinusitis（control group）.Nasal polyps of 94 patients were obtained in endoscopic sinus surgery,HE staining and immunohistochemical SP staining were performed to detect the distribution of eosinophils（EOS） and the expression of COX-2.Statistical analysis was performed using the SPSS software and then the relationship between their expression and distribution with clinical pathology and pathogenesis was analyzed.Result：EOS infiltrated extensively in nasal polyps,and EOS counts in these groups were 80.02±6.11,76.62±5.22,65.97±4.78,respectively.The difference between ST group,ATA group and control group are significant（P0.05）,no significant difference between ST group and ATA group.COX-2 in the nasal polyps was mainly expressed in submucosal glands,glandular epithelial cells,epithelial cells,endothelial cells and EOS in interstitium.The positive cell count were 88.13±6.26,89.46±5.97,91.22±4.11,respectively.There was significant difference（P0.05） between ST group and control group.No significant difference（P0.05） was found between ATA group and control group,ST group and ATA group.Conclusion：The difference of EOS infiltration in patients with ST may be associated with an inflammatory response underlying specific clinical manifestations after exposure to non-steroidal anti-inflammatory drugs,and the difference of COX-2 expression in patients with ST may be related to the conversion of metabolic pathway of arachidonic acid and the formation of nasal polyps.

关 键 词：阿司匹林三联征 鼻息肉 嗜酸粒细胞 COX-2 免疫组织化学 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]